Occurrence of ferredoxin:NAD(+) oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria

PeerJ
Verena HessVolker Müller

Abstract

A ferredoxin:NAD(+) oxidoreductase was recently discovered as a redox-driven ion pump in the anaerobic, acetogenic bacterium Acetobacterium woodii. The enzyme is assumed to be encoded by the rnf genes. Since these genes are present in the genomes of many bacteria, we tested for ferredoxin:NAD(+) oxidoreductase activity in cytoplasmic membranes from several different Gram-positive and Gram-negative bacteria that have annotated rnf genes. We found this activity in Clostridium tetanomorphum, Clostridium ljungdahlii, Bacteroides fragilis, and Vibrio cholerae but not in Escherichia coli and Rhodobacter capsulatus. As in A. woodii, the activity was Na(+)-dependent in C. tetanomorphum and B. fragilis but Na(+)-independent in C. ljungdahlii and V. cholerae. We deleted the rnf genes from B. fragilis and demonstrated that the mutant has greatly reduced ferredoxin:NAD(+) oxidoreductase activity. This is the first genetic proof that the rnf genes indeed encode the reduced ferredoxin:NAD(+) oxidoreductase activity.

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Citations

Apr 29, 2016·Gut Microbes·Patricia G WolfH Rex Gaskins
Jun 21, 2018·Annual Review of Microbiology·Volker MüllerMirko Basen
Mar 7, 2018·Biotechnology for Biofuels·Kaspar ValgepeaEsteban Marcellin
Jun 5, 2019·Biotechnology and Bioengineering·Johannes Schiffels, Thorsten Selmer
Sep 8, 2019·Proceedings of the National Academy of Sciences of the United States of America·Sebastian C TreitliVladimír Hampl
May 31, 2020·Proceedings of the National Academy of Sciences of the United States of America·Vishnuvardhan MahamkaliEsteban Marcellin
Dec 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Marie Charlotte SchoelmerichVolker Müller
Mar 22, 2018·Proceedings of the National Academy of Sciences of the United States of America·Zheng LuJames A Imlay
Jan 6, 2021·Chemical Reviews·Filipa CalistoManuela M Pereira
Feb 21, 2021·Archives of Biochemistry and Biophysics·Kanwal KayasthaUlrich Ermler
Mar 23, 2021·Frontiers in Bioengineering and Biotechnology·Maria HermannRalf Takors
Sep 5, 2021·Extremophiles : Life Under Extreme Conditions·Florian P Rosenbaum, Volker Müller
Jul 15, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Martin KuhnsVolker Müller

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