PMID: 6166471May 1, 1981Paper

Occurrence of heterogenous forms of the subunits of creatine kinase in various muscle and nonmuscle tissues and their behaviour during myogenesis

European Journal of Biochemistry
U B RosenbergJ C Perriard

Abstract

Purified, homodimeric creatine kinases from chicken were subjected to two-dimensional gel analysis under dissociating conditions. Each of the subunits M-creatine kinase and B-creatine kinase was resolved into a basic and an acidic subspecies with very similar mobilities in the sodium dodecylsulfate dimension. The M-creatine kinase subspecies were found in myogenic cells, fast muscle, slow muscle and the B-creatine kinase subspecies were present in heart, gizzard and brain. The creatine kinase subunits were identified in these tissues by a variety of methods like immunoreplicas of two-dimensional gels, immunoprecipitations, or coelectrophoresis with purified creatine kinase and all gave the same results. In the course of myogenic development in vitro the subspecies were synthesized coordinately and no indication was found for a differential regulation of any of the subspecies of the creatine kinase subunits. No radioactive phosphorus was incorporated into either one of the subspecies, hence phosphorylation could be ruled out as the source of heterogeneity. Furthermore, peptide mapping analysis of partial proteolytic digests did not reveal differences among the subspecies of the same subunit. Not only chicken but also rat creatin...Continue Reading

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Citations

Apr 1, 1994·Molecular and Cellular Biochemistry·S M MühlebachM Wyss
May 9, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·B G Guslits, H K Jacobs
Sep 16, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·F Kanemitsu, T Okigaki
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C W SchweinfestR P Dottin
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·U B RosenbergJ C Perriard
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J GiraudatJ P Changeux
Apr 1, 1988·The Journal of Cell Biology·B W Schäfer, J C Perriard
Nov 25, 1991·Nucleic Acids Research·M E RitchieJ J Billadello
Jul 1, 1985·European Journal of Biochemistry·V Witzemann
Dec 1, 1994·The American Journal of the Medical Sciences·A ZemtsovF Mattioli
Jan 1, 1987·Journal of Child Neurology·S T IannacconeF J Samaha
Jun 21, 1990·Biochimica Et Biophysica Acta·R V Trask, J J Billadello

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