PMID: 9438551Jan 23, 1998Paper

Occurrence of oxidatively modified proteins: an early event in experimental acute pancreatitis

Free Radical Biology & Medicine
T ReinheckelW Halangk

Abstract

Free radical-mediated injury is believed to play a key role in the pathogenesis of acute pancreatitis (AP). Therefore, oxidative damage of proteins may be an important event in the development of AP. The present study was performed to investigate oxidative protein modification, quantified as 2,4-dinitrophenylhydrazine-reactive protein-carbonyls, during the time course of taurocholate-induced pancreatitis of the rat and to analyze oxidatively modified proteins by Western blotting. Protein modification in pancreatic homogenates was found as early as 30 min after induction of severe AP with 3% taurocholate preceding the elevation of serum amylase activity and the increase of malondialdehyde in the tissue. A correlation of protein-carbonyl contents to a score of pancreatic macroscopic alterations (r = .69) and to the wet weight/dry weight ratio (r = .65) was found. Infusion of 5% taurocholate resulted in fulminant AP with high lethality during the 24 h of the experiment. However, rats surviving showed significantly lower level of protein-carbonyls than animals that died between 20-24 h after AP induction. The quantitative data were confirmed by the intensity of immunostained protein-carbonyls. The present data show a rather uniform...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Uchida, E R Stadtman
Apr 1, 1988·Mechanisms of Ageing and Development·M PootJ F Jongkind
Jan 1, 1980·Scandinavian Journal of Gastroenterology·H J AhoT J Nevalainen
Aug 1, 1995·Archives of Biochemistry and Biophysics·Y Lee, E Shacter
Jan 1, 1994·Methods in Enzymology·A Z Reznick, L Packer
Jul 1, 1994·Digestive Diseases and Sciences·D ClosaE Gelpi
Jan 1, 1995·Biochemical Society Symposium·K J Davies
Jun 28, 1996·The Journal of Biological Chemistry·T GruneK J Davies
Jan 1, 1996·Free Radical Biology & Medicine·C NiederauD Häussinger
Aug 1, 1996·Free Radical Research·G WaegH Esterbauer

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Citations

Feb 13, 2001·Free Radical Biology & Medicine·P KruseS Loft
Sep 1, 1999·Free Radical Biology & Medicine·A C BlackburnG D Buffinton
Feb 3, 2000·Free Radical Biology & Medicine·C JolivaltG Siest
Mar 7, 2001·Antioxidants & Redox Signaling·M ParolaM U Dianzani
Aug 13, 2011·Antioxidants & Redox Signaling·Thilo Hackert, Jens Werner
Jan 7, 2004·Pancreas·Carmen Sánchez-BernalNieves Pérez-González
Dec 3, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M ChvanovA Tepikin
May 9, 2007·FEBS Letters·Yuki OgasawaraKazuyuki Ishii
May 11, 2004·Mass Spectrometry Reviews·Marina CariniRoberto Maffei Facino
Apr 30, 2014·Cell Biochemistry and Function·Cristina CarrascoJosé A Pariente
Nov 25, 2011·European Journal of Oral Sciences·Mikael SonessonClaes Wickström
Aug 18, 2004·The International Journal of Biochemistry & Cell Biology·Juan Du, Janusz M Gebicki
Jul 16, 2005·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Changbin ShiXiangdong Wang
Aug 20, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Qian WuJian-Ting Chen
Jul 12, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Pinar ErkekogluSteven R Tannenbaum
Aug 21, 2013·Free Radical Research·J A ArmstrongD N Criddle
Oct 1, 2013·The Journal of Nutritional Biochemistry·Tsui-Ling ChangChu-Mei Hong
Sep 11, 2002·The Journal of Nutrition·Kevin A Cockell, Bartholomeus Belonje
Aug 10, 2005·The Biochemical Journal·Thomas NauserJanusz M Gebicki
Jan 25, 2002·Annals of the New York Academy of Sciences·E R Stadtman
Sep 29, 2006·World Journal of Gastroenterology : WJG·Mariette VerlaanJoost P H Drenth
Feb 5, 2019·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yasuhito NobushiKazuo Uchikura
Jan 28, 2006·World Journal of Gastroenterology : WJG·Zhen-Dong LiChang-An Wang
Mar 17, 2018·Frontiers in Immunology·Nicolas S MerleLubka T Roumenina
Apr 16, 2019·Oxidative Medicine and Cellular Longevity·Mateusz MaciejczykAnna Zalewska
Jan 26, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shahram AgahJavad Heshmati
Jan 6, 2000·Life Sciences·D TanN J Farley
Jul 17, 1999·Analytical Chemistry·C K LariveS Bogdanowich-Knipp

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