PMID: 8610115Apr 2, 1996Paper

Occurrence of poly(alpha2,8-deaminoneuraminic acid) in mammalian tissues: widespread and developmentally regulated but highly selective expression on glycoproteins

Proceedings of the National Academy of Sciences of the United States of America
Martin ZiakJ Roth

Abstract

In tissues of higher organisms homopolymers of alpha2,8-linked N-acetylneuraminic acid can be found as a posttranslational modification on selected proteins. We report here the discovery of homopolymers of alpha2,8-linked deaminoneuraminic acid [poly(alpha2,8-KDN)] in various tissues derived from all three germ layers in vertebrates including mammals. The monoclonal antibody kdn8kdn in conjunction with a bacterial KDNase permitted the detection of poly(alpha2,8-KDN) by immunohistochemistry and immunoblotting. Further evidence for the existence of poly(alpha2,8-KDN) was obtained by gas/liquid chromatography. The poly(alpha2,8-KDN) glycan was detectable in all tissues studied with the exception of mucus-producing cells present in various organs, the extracellular matrix, and basement membranes. However, in certain organs such as muscle, kidney, lung, and brain its expression was developmentally regulated. Despite its widespread tissue distribution, the poly(alpha2,8-KDN) glycan was detected on a single 150-kDa glycoprotein except for a single >350-kDa glycoprotein in kidney, which makes it most distinctive among polysialic acids. The ubiquitous yet selective expression may be indicative of a general function of the poly(alpha2,8-...Continue Reading

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Citations

Nov 28, 2000·Carbohydrate Research·P FlorioM von Itzstein
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·B QuJ Roth
Apr 8, 2014·Acta histochemica·Mirca MariniEleonora Sgambati
Jan 5, 2005·Biochemical and Biophysical Research Communications·Thomas HaselhorstMark von Itzstein
Feb 14, 2015·Future Neurology·Xiaohua XuMoses Rodriguez
Aug 3, 2004·Microscopy Research and Technique·Francisco José SáezFrancisco Hernández
Dec 30, 2020·The Journal of Clinical Investigation·Kunio KawanishiAjit Varki
Jul 30, 1999·Biochemical and Biophysical Research Communications·T AngataK Kitajima
Jul 9, 2002·Biochemical and Biophysical Research Communications·Martin ZiakJürgen Roth
Apr 24, 1999·Journal of the American Society of Nephrology : JASN·M ZiakJ Roth
Jul 7, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Mahmoud S GewailyMohamed Kassab

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