Occurrence of tetraploidy in Nicotiana attenuata plants after Agrobacterium-mediated transformation is genotype specific but independent of polysomaty of explant tissue

Plant Cell Reports
Ben BubnerI T Baldwin

Abstract

Genotypes of Nicotiana attenuata collected from Utah and Arizona were transformed with 17 different vectors (14 unpublished vectors based on 3 new backbone vectors) using an Agrobacterium-mediated procedure to functionally analyze genes important for plant-insect interactions. None of the 51 T1-T3 transgenic Utah lines analyzed by the flow cytometry were tetraploid, as opposed to 18 of 33 transgenic Arizona lines (55%). Analysis of T0 regenerants transformed with the same vector carrying an inverted repeat (IR) N. attenuata pro-systemin construct confirmed the genotype dependency of tetraploidization: none of the 23 transgenic Utah lines were tetraploid but 31 (72%) of 43 transgenic Arizonas were tetraploid. We tested the hypothesis that the differences in polysomaty of the explant tissues accounted for genotype dependency of tetraploid formation by measuring polysomaty levels in different seedling tissues. Hypocotyls, cotyledons, and roots of Utah and Arizona genotypes contained similar percentages of 4C nuclei (61 and 60; 7 and 5; and 58 and 61%, respectively). Since we used hypocotyls as explant sources and the nonoccurrence of tetraploid Utah transformants does not correspond to the high percentage of 4C nuclei in Utah hypo...Continue Reading

References

Nov 23, 2000·Plant Molecular Biology·J Joubès, C Chevalier
Feb 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jorge A ZavalaIan T Baldwin
Nov 1, 1950·Proceedings of the National Academy of Sciences of the United States of America·H SWIFT
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Dec 1, 1990·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R W van den BulkM Koornneef

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Citations

May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mario KallenbachIan T Baldwin
Feb 27, 2008·The Plant Journal : for Cell and Molecular Biology·Shree P Pandey, Ian T Baldwin
Mar 10, 2007·The Plant Journal : for Cell and Molecular Biology·Shree P Pandey, Ian T Baldwin
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Caroline C von DahlIan T Baldwin
Jun 10, 2006·The Plant Journal : for Cell and Molecular Biology·Ian S PearseIan T Baldwin
Mar 28, 2012·The Plant Journal : for Cell and Molecular Biology·Danny KesslerIan T Baldwin
Aug 7, 2012·The Plant Journal : for Cell and Molecular Biology·Melkamu G WoldemariamIvan Galis
Apr 27, 2011·Molecular Ecology Resources·Klaus GaseIan T Baldwin
Jun 23, 2015·The Plant Journal : for Cell and Molecular Biology·Michele BandolyAnke Steppuhn
Mar 25, 2016·Proceedings. Biological Sciences·Meret HuberMatthias Erb
Jul 2, 2015·ELife·Danny KesslerIan T Baldwin
May 6, 2016·ELife·Alexander HaverkampDanny Kessler
Apr 22, 2017·Journal of Integrative Plant Biology·Youngsung JooSang-Gyu Kim

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