OCEAN-C: mapping hubs of open chromatin interactions across the genome reveals gene regulatory networks

Genome Biology
Tingting LiCheng Li


We develop a method called open chromatin enrichment and network Hi-C (OCEAN-C) for antibody-independent mapping of global open chromatin interactions. By integrating FAIRE-seq and Hi-C, OCEAN-C detects open chromatin interactions enriched by active cis-regulatory elements. We identify more than 10,000 hubs of open chromatin interactions (HOCIs) in human cells, which are mainly active promoters and enhancers bound by many DNA-binding proteins and form interaction networks crucial for gene transcription. In addition to identifying large-scale topological structures, including topologically associated domains and A/B compartments, OCEAN-C can detect HOCI-mediated chromatin interactions that are strongly associated with gene expression, super-enhancers, and broad H3K4me3 domains.


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Datasets Mentioned


Methods Mentioned

Illumina sequencing

Related Concepts

DNA Helix Destabilizing Proteins
Enhancer Elements, Genetic
Early Promoters, Genetic
Histone Code
Cell Line, Tumor
Gene Modules
Body Fluid Compartments
DNA-Binding Proteins

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