Ochratoxin A biosynthetic genes in Aspergillus ochraceus are differentially regulated by pH and nutritional stimuli

Fungal Genetics and Biology : FG & B
John O'CallaghanAlan D W Dobson

Abstract

Expression of the polyketide synthase (pks) gene which is involved in ochratoxin A (OTA) biosynthesis in Aspergillus ochraceus is linked to production of the mycotoxin, with high levels of pks mRNA accumulation occurring in cultures producing OTA, as assessed by real-time reverse transcription (RT)-PCR. OTA production is regulated by nutrient availability, with supplementation of OTA restrictive potato dextrose broth with yeast extract resulting in a 39-fold increase in production of the mycotoxin. This effect appears to be mediated at the level of gene transcription as there is a concomitant increase in pks mRNA accumulation. OTA production is also strongly influenced by culture pH with large amounts of OTA being produced at pH values <7.0 with reduced amounts being produced at higher pH values. pks transcript levels again mirrored the OTA production profile observed at the different pH values. The transcription of two putative p450 type monooxygenase genes, namely p450-H11 and p450-B03 genes closely mirrored that of the pks gene under all growth conditions tested, suggesting their involvement together with the pks in OTA biosynthesis. The expression profile of the p450-B03 gene in particular is very similar to that of the pks...Continue Reading

References

Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W BrownT J Leonard
Sep 29, 1998·Symposium Series·M O Moss
Nov 26, 1998·Food Additives and Contaminants·K Jørgensen
Jun 10, 2000·Journal of Veterinary Pharmacology and Therapeutics·E Petzinger, K Ziegler
Feb 24, 2001·Medical Mycology·J I Pitt
May 8, 2001·Food Additives and Contaminants·M A SkaugO D Saugstad
Oct 24, 2001·Phytochemistry·J P Harris, P G Mantle
Nov 7, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A ThuvanderM Olsen
Jan 29, 2002·Food Additives and Contaminants·B Odhav, V Naicker
Apr 27, 2002·Applied and Environmental Microbiology·Paul BaymanNoreen E Mahoney
Jun 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Todd J WardKerry O'Donnell
Mar 8, 2003·Food Additives and Contaminants·K Jørgensen, J S Jacobsen
Mar 26, 2003·Applied Microbiology and Biotechnology·D BhatnagarT E Cleveland
May 7, 2003·Applied and Environmental Microbiology·Andrew W PeplowMarian N Beremand
Jul 25, 2003·International Journal of Food Microbiology·Luis Roberto BatistaAlan E Wheals
Sep 6, 2003·Applied and Environmental Microbiology·Joseph E FlahertyCharles P Woloshuk
Oct 7, 2003·Research in Microbiology·János VargaKároly Pál
Nov 25, 2003·Journal of Applied Microbiology·F Lund, J C Frisvad
Jan 17, 2004·Food Additives and Contaminants·K A ScudamoreS J MacDonald
Mar 10, 2004·Applied and Environmental Microbiology·Jiujiang YuJoan W Bennett
Nov 6, 2004·Applied and Environmental Microbiology·Kenneth C EhrlichPeter J Cotty
Feb 1, 2005·International Journal of Food Microbiology·M BauF J Cabañes
Feb 15, 2005·Clinical Biochemistry·Srebrena Y AtanasovaVictor W Armstrong

❮ Previous
Next ❯

Citations

Mar 1, 2007·Mycotoxin Research·M Schmidt-Heydt, R Geisen
Jan 27, 2010·Applied Microbiology and Biotechnology·Massimo ReverberiAnna Adele Fabbri
May 25, 2010·Applied Microbiology and Biotechnology·Massimo ReverberiCorrado Fanelli
Mar 14, 2012·Applied Microbiology and Biotechnology·Massimo ReverberiCorrado Fanelli
Jul 1, 2011·The Journal of Microbiology·Wenbing Yin, Nancy P Keller
Apr 1, 2010·Toxins·André el Khoury, Ali Atoui
Dec 4, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Eva M MateoM Jimenez
Oct 28, 2015·Toxins·Alexandra H Heussner, Lewis E H Bingle
Jan 22, 2013·International Journal of Food Microbiology·Abdelhamid AbbasAlan D W Dobson
Feb 22, 2012·International Journal of Food Microbiology·Lara Munique FerracinMaria Helena Pelegrinelli Fungaro
Sep 9, 2011·International Journal of Food Microbiology·Jéssica Gil-SernaCovadonga Vázquez
Aug 2, 2011·International Journal of Food Microbiology·Alicia RodríguezJuan J Córdoba
May 3, 2011·International Journal of Food Microbiology·A Crespo-SempereP V Martínez-Culebras
Aug 11, 2015·International Journal of Food Microbiology·Jessica Gil-SernaBelén Patiño
Jun 6, 2009·Microbiology·Donald M GardinerJohn M Manners
Jan 21, 2016·Journal of Applied Microbiology·B F CarvalhoR F Schwan
Sep 18, 2013·International Journal of Food Microbiology·A Crespo-SempereA J Ramos
May 31, 2008·FEMS Microbiology Letters·Markus Schmidt-HeydtRolf Geisen
Apr 23, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Cristina JuanJordi Mañes
May 26, 2017·Journal of Agricultural and Food Chemistry·Jesús M González-Jartı NLuis M Botana
Jul 17, 2016·Applied and Environmental Microbiology·Massimo FerraraAntonia Gallo
Oct 3, 2007·Journal of Basic Microbiology·Ganesh SarataleSanjay Govindwar
Mar 29, 2007·Natural Product Reports·Dirk Hoffmeister, Nancy P Keller
May 15, 2007·Canadian Journal of Microbiology·Marcia M MataMaria Helena P Fungaro
Oct 13, 2016·Annual Review of Genetics·Juliane MacheleidtAxel A Brakhage
Nov 17, 2021·Journal of Agricultural and Food Chemistry·Melvin S SamuelEthiraj Selvarajan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.