Ochratoxin A increases permeability through tight junctions by removal of specific claudin isoforms

American Journal of Physiology. Cell Physiology
John McLaughlinCatherine A O'Neill

Abstract

On interaction with the intestine, the mycotoxin ochratoxin A is know to cause rapid inflammation, diarrhea, and increased bacterial translocation. All these effects are consistent with a decrease in epithelial barrier function. However, this has not been shown directly. We determined that ochratoxin A is able to reduce the barrier properties of the model intestinal cell line Caco-2. Over 24 h, ochratoxin A reduces the transepithelial electrical resistance of Caco-2 monolayers growing on Transwell filters by approximately 40%. At the same time, the permeability of the monolayer is increased with respect to 4- and 10-kDa FITC dextrans, but not to 20- or 40-kDa dextrans. Immunoblotting and immunofluorescence reveal that the decrease in barrier properties is concomitant with disappearance of claudins 3 and 4, but not claudin 1 from Caco-2 cell membranes. These results suggest that ochratoxin A is able to modulate the barrier function of Caco-2 cells by removal of specific claudin isoforms.

References

Dec 1, 1992·Journal of Animal Science·R R Marquardt, A A Frohlich
Nov 1, 1989·JPEN. Journal of Parenteral and Enteral Nutrition·S InoueP Gura
Dec 1, 1968·Food and Cosmetics Toxicology·I F Purchase, J J Theron
Nov 1, 1980·The American Journal of Clinical Nutrition·R D Berg
Jun 1, 1984·Bulletin of Environmental Contamination and Toxicology·L F KubenaN D Heidelbaugh
May 1, 1995·Gastroenterology·I BjarnasonD Hollander
Dec 1, 1993·The Journal of Cell Biology·M FuruseS Tsukita
Jan 1, 1996·Food Additives and Contaminants·P M BolgerC D Carrington
Jun 16, 1997·The Journal of Cell Biology·A SakakibaraS Tsukita
Apr 4, 2000·The Journal of Cell Biology·S Tsukita, M Furuse

❮ Previous
Next ❯

Citations

Feb 16, 2006·The Journal of Membrane Biology·L González-MariscalS Hernández
May 15, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Anke L L LamerisJoost G J Hoenderop
Dec 25, 2012·Therapeutic Delivery·Bharti SapraAshok K Tiwary
May 27, 2014·Toxins·Philippe Pinton, Isabelle P Oswald
May 15, 2010·Toxicon : Official Journal of the International Society on Toxinology·Marc Maresca, Jacques Fantini
Nov 7, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Eric S ClellandScott P Kelly
Sep 2, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G RanaldiC Murgia
Oct 28, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C A González-AriasA J Ramos
Dec 17, 2010·The Journal of Investigative Dermatology·Takuo YukiShintaro Inoue
Nov 18, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·John McLaughlinCatherine A O'Neill
Mar 16, 2007·Experimental Dermatology·Takuo YukiShintaro Inoue
May 25, 2013·Journal of Veterinary Pharmacology and Therapeutics·J GoossensS Croubels
Jun 29, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Ozkan OzdenBrenda J Grubb
May 15, 2007·Biochemical and Biophysical Research Communications·Daniel LambertCatherine A O'Neill
Nov 17, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G RanaldiG Perozzi
Sep 8, 2005·Veterinary Immunology and Immunopathology·Sandrine Bouhet, Isabelle P Oswald
Jun 24, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G Cano-SanchoM L Fernández-Cruz
Dec 17, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Akihiro WatariMasuo Kondoh
Aug 22, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H M MohanA W Baird
Jul 28, 2009·Environmental Pollution·Erica DonnerStephen Nortcliff
Apr 22, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nolwenn HymeryEmmanuel Coton
May 9, 2012·Regulatory Peptides·G W MoranJ T McLaughlin
Mar 18, 2009·Toxicology and Applied Pharmacology·Philippe PintonIsabelle P Oswald
Dec 6, 2014·Biochemical and Biophysical Research Communications·Sehwan ShimSunhoo Park
Jun 21, 2016·Advanced Drug Delivery Reviews·Sam MaherDavid J Brayden
Jul 16, 2016·Archives of Toxicology·Peyman AkbariJohanna Fink-Gremmels
Aug 9, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ricardo AssunçãoPaula Alvito
Sep 9, 2016·Frontiers in Cellular and Infection Microbiology·Paul Ugalde-SilvaFernando Navarro-Garcia
Apr 24, 2010·Human & Experimental Toxicology·Hassan MalekinejadFereshteh Dardmeh
Nov 18, 2004·Physiology·Christina M Van Itallie, James Melvin Anderson
Jun 22, 2007·The Journal of Pathology·R E B WatsonC A O'neill
Dec 19, 2018·The Journal of Experimental Biology·Julia GaubergCraig E Franklin

❮ Previous
Next ❯

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