Oct-1 and nuclear factor Y bind to the SURG-1 element to direct basal and gonadotropin-releasing hormone (GnRH)-stimulated mouse GnRH receptor gene transcription

Molecular Endocrinology
Kyung-Yoon KamUrsula B Kaiser

Abstract

The cis-regulatory element localized to position -292/-285 of the mouse GnRH receptor (mGnRHR) gene promoter, designated Sequence Underlying Responsiveness to GnRH 1 (SURG-1), has been shown previously to contribute to stimulation of mGnRHR gene expression by GnRH. We have identified three specific protein-DNA complexes on the SURG-1 element by EMSA using nuclear extracts from the gonadotrope-derived alphaT3-1 and LbetaT2 cell lines. Serial mutagenesis and supershift assays identified nuclear factor Y (NF-Y) binding to -288/-284 and Oct-1 binding to a TAAT sequence at -290/-287. Binding of these two transcription factors was confirmed in vivo by chromatin immunoprecipitation assay and increased in response to GnRH stimulation. To define the functional significance of these sequences in the regulation of mGnRHR gene transcription, transient transfection assays were performed in alphaT3-1 cells using a 1.2-kb mGnRHR (-1164/+62) gene promoter-luciferase reporter construct with selective mutations of the Oct-1, NF-Y, and/or the previously characterized activating protein 1 (AP-1) binding site (-274/-268). Individual mutations in the Oct-1, NF-Y, and AP-1 sites decreased both basal expression and stimulation by GnRH agonist, and the...Continue Reading

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Citations

Oct 16, 2009·Journal of Virology·Anna S KushnirPriscilla A Schaffer
Feb 12, 2005·Endocrinology·Shauna M McGillivrayPamela L Mellon
Jun 14, 2008·Endocrine-related Cancer·Cuiqi ZhouShlomo Melmed
Sep 15, 2005·Journal of Neuroendocrinology·J P HapgoodK Ronacher
Jan 8, 2009·Journal of Cellular Biochemistry·Jin-Xing QuanBao-Li Wang
Feb 6, 2007·Biochemical and Biophysical Research Communications·Zhaoliang FeiJian Fei
Jun 9, 2006·Molecular Systems Biology·Debopriya DasMichael Q Zhang
Jun 9, 2016·Molecular Endocrinology·Alice K TreenDenise D Belsham

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