Oct-1 binds promoter elements required for transcription of the GnRH gene

Molecular Endocrinology
S A EralyP L Mellon

Abstract

The GnRH gene is exclusively expressed in a discrete population of neurons in the hypothalamus. The promoter-proximal 173 bp of the rat GnRH gene are highly conserved through evolution and are bound by multiple nuclear proteins found in the neuronal cell line, GT1-7, a model for the GnRH-expressing hypothalamic neuron. To explore the protein-DNA interactions that occur within this promoter and the role of these interactions in targeting GnRH gene expression, we have mutagenized individual binding sites in this region. Deoxyribonuclease I protection experiments reveal that footprint 2, a 51-bp sequence that confers a 20-fold induction of the GnRH gene, is comprised of at least three independent protein-binding sites. Transfections of the GnRH promoter-reporter plasmid containing a series of block mutations of footprint 2 into GT1-7 neurons indicate that each of the three putative component sites contributes to transcriptional activity. Mutations in footprint 4 also decrease GnRH gene expression. Footprint 4 and the promoter-proximal site in footprint 2 contain octamer-like motifs, an element that is also present in the neuron-specific enhancer of the rat GnRH gene located approximately 1.6 kb upstream of the promoter. Previous s...Continue Reading

References

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Citations

Dec 10, 2009·American Journal of Physiology. Endocrinology and Metabolism·Sheng ZhaoRussell D Fernald
Nov 15, 2011·Molecular Endocrinology·Melissa J BraymanPamela L Mellon
Dec 1, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Horacio J NovairaSally Radovick
Jun 14, 2008·Endocrine-related Cancer·Cuiqi ZhouShlomo Melmed
Feb 9, 2013·Molecular Endocrinology·Christine A Glidewell-KenneyPamela L Mellon
Jun 18, 2015·Molecular Neurobiology·H J NovairaS Radovick
Sep 1, 2005·Journal of Biomedical Science·Ming-Wei KuoBon-Chu Chung
Aug 29, 2002·Molecular Endocrinology·Rafael Vazquez-MartinezFredric R Boockfor
Jun 9, 2016·Molecular Endocrinology·Alice K TreenDenise D Belsham
Sep 24, 2010·Endocrinology·Joseph R KurianEi Terasawa
Jan 5, 2002·The Journal of Biological Chemistry·Wenhong FanQimin Zhan
Jul 25, 2003·Biochemical and Biophysical Research Communications·Christopher M MayerDenise D Belsham

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