Oct-1 cooperates with the TATA binding initiation complex to control rapid transcription of human iNOS.

Cellular and Molecular Life Sciences : CMLS
Sylvie ReveneauAlena Pance

Abstract

Expression of the human inducible nitric oxide synthase (hiNOS) is generally undetectable in resting cells, but stimulation by a variety of signals including cytokines induces transcription in most cell types. The tight transcriptional regulation of the enzyme is a complex mechanism many aspects of which remain unknown. Here, we describe an octamer (Oct) element in hiNOS proximal promoter, located close to the TATA box. This site constitutively binds Oct-1 and its deletion abrogates cytokine-induced transcription, showing that it is indispensable though not sufficient for transcription. Increasing the distance between Oct and the TATA box by inserting inert DNA sequence inhibits transcription, and footprinting of this region shows no other protein binding in resting cells, suggesting an interaction between the two complexes. Chromatin immunoprecipitation assays detect the presence of Oct-1, RNA polymerase II and trimethyl K4 histone H3 on the proximal promoter in resting cells, confirming that the gene is primed for transcription before stimulation. RT-PCR of various fragments along the hiNOS gene shows that transcription is initiated in resting cells and this is inhibited by interference with Oct-1 binding to the proximal site...Continue Reading

References

Nov 17, 1995·The Journal of Biological Chemistry·M H Kim, D O Peterson
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C J LowensteinW J Murphy
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·M E de VeraD A Geller
Dec 11, 1999·Nucleic Acids Research·E WingenderF Schacherer
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C E GoldringJ F Jeannin
Feb 24, 2001·Journal of Molecular Biology·K Phillips, B Luisi
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·R W GansterD A Geller
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alena PanceJean-François Jeannin
Aug 23, 2002·Molecular Cell·Eric Bertolino, Harinder Singh
Sep 28, 2002·Nature·Helena Santos-RosaTony Kouzarides
Jul 1, 2005·Nature·Tae Hoon KimBing Ren
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gary C ChanPhilip A Marsden
Aug 29, 2006·Nature Reviews. Molecular Cell Biology·Abbie SaundersJohn T Lis
Jul 3, 2007·Nature·Tarjei S MikkelsenBradley E Bernstein
Aug 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Roger D Kornberg
Nov 13, 2007·Nature Genetics·Ginger W MuseKaren Adelman
May 20, 2008·Cell·Thanasis Margaritis, Frank C P Holstege
Oct 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Karen AdelmanInez Rogatsky
Apr 7, 2010·Annual Review of Biochemistry·Luke A SelthJesper Q Svejstrup

❮ Previous
Next ❯

Citations

Apr 12, 2016·Biochimica Et Biophysica Acta·Alena Pance
Feb 16, 2016·Biochimica Et Biophysica Acta·Karina Vázquez-Arreguín, Dean Tantin
Apr 10, 2013·International Journal of Molecular Sciences·Alena Pance
Jun 29, 2021·Frontiers in Cellular and Infection Microbiology·Marina de Assis SouzaHiro Goto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.