Oct3/4 directly regulates expression of E2F3a in mouse embryonic stem cells

Biochemical and Biophysical Research Communications
Dai KanaiHiroshi Koide

Abstract

Embryonic stem (ES) cells, derived from the inner cell mass of blastocysts, have a characteristic cell cycle with truncated G1 and G2 phases. Recent findings that suppression of Oct3/4 expression results in a reduced proliferation rate of ES cells suggest the involvement of Oct3/4 in the regulation of ES cell growth, although the underlying molecular mechanism remains unclear. In the present study, we identified E2F3a as a direct target gene of Oct3/4 in ES cells. Oct3/4 directly bound to the promoter region of the E2F3a gene and positively regulated expression of E2F3a in mouse ES cells. Suppression of E2F3a activity by E2F6 overexpression led to the reduced proliferation in ES cells, which was relieved by co-expression of E2F3a. Furthermore, cell growth retardation caused by loss of Oct3/4 was rescued by E2F3a expression. These results suggest that Oct3/4 upregulates E2F3a expression to promote ES cell growth.

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Citations

Nov 2, 2015·Current Opinion in Cell Biology·Michael S KaretaMarius Wernig
Nov 18, 2017·ELife·Jacob Peter MatsonJeanette Gowen Cook
Mar 9, 2017·Cancer Biomarkers : Section a of Disease Markers·Hala Abdel Al Ahmed, Ola Nada
Sep 14, 2017·Molecular Medicine Reports·Shiqi SheYing-Jie Wang
Jul 6, 2018·Frontiers in Cell and Developmental Biology·Lamuk Zaveri, Jyotsna Dhawan

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