Oct4/Sox2-regulated miR-302 targets cyclin D1 in human embryonic stem cells.

Molecular and Cellular Biology
Deborah A Greer CardTrevor K Archer

Abstract

Oct4 and Sox2 are transcription factors required for pluripotency during early embryogenesis and for the maintenance of embryonic stem cell (ESC) identity. Functional mechanisms contributing to pluripotency are expected to be associated with genes transcriptionally activated by these factors. Here, we show that Oct4 and Sox2 bind to a conserved promoter region of miR-302, a cluster of eight microRNAs expressed specifically in ESCs and pluripotent cells. The expression of miR-302a is dependent on Oct4/Sox2 in human ESCs (hESCs), and miR-302a is expressed at the same developmental stages and in the same tissues as Oct4 during embryogenesis. miR-302a is predicted to target many cell cycle regulators, and the expression of miR-302a in primary and transformed cell lines promotes an increase in S-phase and a decrease in G(1)-phase cells, reminiscent of an ESC-like cell cycle profile. Correspondingly, the inhibition of miR-302 causes hESCs to accumulate in G(1) phase. Moreover, we show that miR-302a represses the productive translation of an important G(1) regulator, cyclin D1, in hESCs. The transcriptional activation of miR-302 and the translational repression of its targets, such as cyclin D1, may provide a link between Oct4/Sox2 an...Continue Reading

References

Jan 1, 1994·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·R KnüppelE Wingender
Nov 13, 2001·Developmental Cell·G FuhrmannA J Cooney
Apr 1, 2003·Molecular and Cellular Biology·Yoshimi TokuzawaShinya Yamanaka
Aug 16, 2003·Developmental Cell·Hristo B HoubaviyPhillip A Sharp
Dec 31, 2003·Cell·Benjamin P LewisChristopher B Burge
Jun 9, 2004·Developmental Biology·Mi-Ra SuhKye-Seong Kim
Sep 17, 2004·The EMBO Journal·Yoontae LeeV Narry Kim
Nov 24, 2004·The Journal of Biological Chemistry·Sayaka Okumura-NakanishiFuyuki Ishikawa
Mar 19, 2005·Science·Antonio J GiraldezAlexander F Schier
Apr 30, 2005·The Journal of Biological Chemistry·David J RoddaPaul Robson
Jun 10, 2005·Nature·Kathryn A O'DonnellJoshua T Mendell
Jun 10, 2005·Nature·S D HatfieldH Ruohola-Baker
Aug 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth P MurchisonGregory J Hannon
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Oct 22, 2005·Stem Cells·Anne-Catherine FluckigerColette Dehay
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Cecilia ConacoGail Mandel
Feb 18, 2006·Science·Antonio J GiraldezAlexander F Schier
May 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Prakash K RaoHarvey F Lodish
Jan 24, 2007·Molecular and Cellular Biology·Peter S LinsleyLee Lim

❮ Previous
Next ❯

Citations

Jun 12, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Nils PfaffTobias Cantz
Sep 22, 2010·Molecular Biology Reports·Haiying LiuYongmei Guan
Oct 26, 2013·Current Atherosclerosis Reports·Xinghui SunMark W Feinberg
Jun 19, 2010·Journal of Cardiovascular Translational Research·Xiaoxiao Zhang, Yan Zeng
Nov 8, 2011·Protein & Cell·Peizhe Wang, Jie Na
Oct 15, 2013·Acta Pharmacologica Sinica·Zheng-ming WangYaguang Xi
Feb 9, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Robert StraussJiri Bartek
Nov 8, 2012·Nature Cell Biology·Trevor R LeonardoLouise C Laurent
Jun 3, 2010·Nature Reviews. Rheumatology·Ilias Alevizos, Gabor G Illei
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sang In LeeJae Yong Han
Feb 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Guiying WangJiuhong Kang
Nov 3, 2011·Antioxidants & Redox Signaling·Ji Woong Han, Young-sup Yoon
Feb 5, 2010·Stem Cells and Development·Bradford StadlerHannele Ruohola-Baker
Jan 23, 2013·Stem Cells and Development·Raquel CalloniDiego Bonatto
Jun 26, 2010·Nucleic Acids Research·Igor UlitskyRon Shamir
Sep 28, 2010·Nucleic Acids Research·Shi-Lung LinDavid T S Wu
Dec 17, 2010·Genes & Development·Gustavo Tiscornia, Juan Carlos Izpisúa Belmonte
Mar 20, 2010·Development, Growth & Differentiation·Kazutoshi Takahashi
May 14, 2009·Cancer Research·Yangming Wang, Robert Blelloch
Mar 25, 2010·Cancer Research·Roger D PalmerUNKNOWN Children's Cancer and Leukaemia Group
Dec 16, 2010·Stem Cell Research & Therapy·Guilai Shi, Ying Jin
Feb 26, 2011·Development·Ying TianEdward E Morrisey
Oct 19, 2012·Journal of Cell Science·Frederick Anokye-DansoEdward E Morrisey
Jan 12, 2013·PloS One·Jenny A WatsonMaureen O'Sullivan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.