PMID: 8972869Dec 1, 1996Paper

Octamer independent activation of transcription from the kappa immunoglobulin germline promoter

Nucleic Acids Research
A PrabhuB G Van Ness

Abstract

Previous analyses of immunoglobulin V region promoters has led to the discovery of a common octamer motif which is functionally important in the tissue-specific and developmentally regulated transcriptional activation of immunoglobulin genes. The germline promoters (Ko) located upstream of the J region gene segments of the kappa locus also contain an octamer motif (containing a single base pair mutation and referred to as the variant octamer) which has been shown previously to bind Oct-1 and Oct-2 transcription factors in vitro. To further elucidate the role of this variant octamer motif in the regulation of germline transcription from the unrearranged kappa locus, we have quantitated the relative binding affinity of Oct-1 and Oct-2 for the variant octamer motif and determined the functional role of this octamer motif in transcriptional activation. We find that, although the variant octamer motif binds Oct-1 and Oct-2 in vitro with 5-fold lower affinity than the consensus octamer motif, mutation of the variant octamer motif to either a consensus octamer or non-octamer motif has no effect on transcriptional activation from the germline promoter. We also find significant differences in activation of germline and V region promoter...Continue Reading

References

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Citations

Sep 17, 2005·Molecular and Cellular Biology·Thibault MesplèdeSébastien Navarro
Mar 21, 2002·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Ana-Isabel SáezMiguel A Piris

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