OCTN2-Mediated Acetyl-l-Carnitine Transport in Human Pulmonary Epithelial Cells In Vitro

Pharmaceutics
Johanna J SalomonCarsten Ehrhardt

Abstract

The carnitine transporter OCTN2 is associated with asthma and other inflammatory diseases. The aims of this work were (i) to determine carnitine uptake into freshly isolated human alveolar type I (ATI)-like epithelial cells in primary culture, (ii) to compare the kinetics of carnitine uptake between respiratory epithelial in vitro cell models, and (iii) to establish whether any cell line was a suitable model for studies of carnitine transport at the air-blood barrier. Levels of time-dependent [3H]-acetyl-l-carnitine uptake were similar in ATI-like, NCl-H441, and Calu-3 epithelial cells, whereas uptake into A549 cells was ~5 times higher. Uptake inhibition was more pronounced by OCTN2 modulators, such as l-Carnitine and verapamil, in ATI-like primary epithelial cells compared to NCl-H441 and Calu-3 epithelial cells. Our findings suggest that OCTN2 is involved in the cellular uptake of acetyl-l-carnitine at the alveolar epithelium and that none of the tested cell lines are optimal surrogates for primary cells.

References

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Citations

Feb 7, 2020·PloS One·Bianca Maria RotoliValeria Dall'Asta
Sep 27, 2020·Pharmaceutics·Ayca Yıldız-Peköz, Carsten Ehrhardt
Dec 5, 2020·International Journal of Molecular Sciences·Mohammed Ali SeloJohanna J Salomon
Jul 14, 2021·Advanced Drug Delivery Reviews·Mohammed Ali SeloCarsten Ehrhardt

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Methods Mentioned

BETA
Protein Assay

Software Mentioned

SigmaPlot
WinNonlin

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