OCTN3: A Na+-independent L-carnitine transporter in enterocytes basolateral membrane

Journal of Cellular Physiology
J M DuránA A Ilundáin

Abstract

L-carnitine transport has been measured in enterocytes and basolateral membrane vesicles (BLMV) isolated from chicken intestinal epithelia. In the nominally Na+-free conditions chicken enterocytes take up L-carnitine until the cell to medium L-carnitine ratio is 1. This uptake was inhibited by L-carnitine, D-carnitine, gamma-butyrobetaine, acetylcarnitine, tetraethylammonium (TEA), and betaine. L-3H-carnitine uptake into BLMV showed no overshoot, and it was (i) Na+-independent, (ii) trans-stimulated by intravesicular L-carnitine, and (iii) cis-inhibited by TEA and cold L-carnitine. L-3H-carnitine efflux from L-3H-carnitine preloaded enterocytes was also Na+-independent, and trans-stimulated by L-carnitine, D-carnitine, gamma-butyrobetaine, acetylcarnitine, TEA, and betaine. Both, uptake and efflux of L-carnitine were inhibited by verapamil and unaffected by either extracellular pH or palmitoyl-L-carnitine. RT-PCR with specific primers for the mouse OCTN3 transporter revealed the existence of OCTN3 mRNA in mouse intestine, which was confirmed by in situ hybridization studies. Immunohystochemical analysis showed that OCTN3 protein was mainly associated with the basolateral membrane of rat and chicken enterocytes, whereas OCTN2 wa...Continue Reading

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Citations

Jan 28, 2006·The Journal of Membrane Biology·P García-MirandaA A Ilundáin
Jan 28, 2010·Pharmacological Reviews·Curtis D Klaassen, Lauren M Aleksunes
Mar 23, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Pascale CarrièreCatherine Jumarie
Mar 27, 2009·Journal of Animal Physiology and Animal Nutrition·B D LambertM G Sanderford
Jan 30, 2010·Journal of Cellular Physiology·M M CanoA A Ilundain
Jul 6, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Tomoko SugiuraYukio Kato
Nov 29, 2007·The Journal of Pharmacology and Experimental Therapeutics·Gerard MinuesaJavier Martinez-Picado
Jun 19, 2013·Journal of Biomolecular Screening·Lorena PochiniCesare Indiveri
Dec 19, 2006·The Biochemical Journal·Wouter F VisserRonald J A Wanders

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