Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus

Plant Molecular Biology
L S MelchersPaul J J Hooykaas

Abstract

Octopine and nopaline strains of Agrobacterium tumefaciens were found to differ in virulence on Nicotiana glauca. This difference is due to the absence of a functional virF locus, which is necessary for efficient tumorigenesis on N. glauca, from the nopaline Ti plasmids. Genetic studies and DNA sequence analysis of the virF locus revealed that virF embraces one open reading frame coding for a hydrophilic protein with a molecular mass of 22,437 Da. Transcription of virF is directed from left to right, towards the T region, and is strongly induced by the phenolic compound acetosyringone. We established that virA and virG, two genes known to be essential for induction of the vir regulon, are necessary for acetosyringone-induced virF expression, implying that virF is a member of this vir regulon. Agrobacterium virF mutants can be complemented for tumor induction by co-infection with avirulent Agrobacterium 'helper' strains. We found that such 'helper' strains must express not only the virF gene but also the vir operons virA, virB, virD and virG.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
May 25, 1988·Nucleic Acids Research·D V ThompsonP J Hooykaas
Sep 1, 1988·Journal of Bacteriology·S C WinansE W Nester
Mar 22, 1985·Science·D J Lipman, W R Pearson
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S C WinansE W Nester
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C GietlB Hohn
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Nov 1, 1985·Science·W G WeisburgE Weiss
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Sep 1, 1980·Journal of Bacteriology·P J HooykaasR A Schilperoort
Jan 1, 1987·Plant Molecular Biology·H P SpainkB J Lugtenberg

❮ Previous
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Citations

May 1, 1992·Plant Molecular Biology·P J Hooykaas, R A Schilperoort
Sep 1, 1989·Plant Molecular Biology·P J Hooykaas
Jul 1, 1996·World Journal of Microbiology & Biotechnology·K Weising, G Kahl
Mar 31, 2004·Fungal Genetics and Biology : FG & B·C B MichielseC A M J J van den Hondel
Aug 6, 2002·Trends in Microbiology·Markus KalkumErich Lanka
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G J PazourA Das
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E JarchowB Hohn
Nov 30, 2006·Nucleic Acids Research·Fernando M García-RodríguezPaul J J Hooykaas
May 15, 2009·Plant Physiology·Stanton B Gelvin
Oct 20, 2011·Science Signaling·Shimpei Magori, Vitaly Citovsky
Jun 27, 2000·Journal of Bacteriology·J ZhuS C Winans
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Stanton B Gelvin
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Stanton B. Gelvin
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BohneA N Binns
Mar 27, 2010·Annual Review of Phytopathology·Stanton B Gelvin
Jul 24, 2014·Molecular Plant-microbe Interactions : MPMI·Arsheed Hussain SheikhAlok Krishna Sinha
Aug 12, 2006·Canadian Journal of Physiology and Pharmacology·Benoît LacroixVitaly Citovsky
May 9, 1998·Molecular Plant-microbe Interactions : MPMI·B SchrammeijerP J Hooykaas
Jun 10, 2009·Seminars in Cell & Developmental Biology·Vitaly CitovskyAlexander Krichevsky
Sep 1, 1992·Plasmid·J C GérardL Otten
Nov 21, 2015·Scientific Reports·Benoît Lacroix, Vitaly Citovsky
Jan 23, 2015·Frontiers in Plant Science·Eugene W Nester
Sep 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Xiaorong ZhangPaul J J Hooykaas
Aug 12, 2018·The Plant Journal : for Cell and Molecular Biology·Mohammad Reza RoushanGerard Paul H van Heusden
Aug 1, 1991·Molecular & General Genetics : MGG·S OkamotoY Machida

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