Ocular Surface Reconstruction with the Autologous Conjunctival Epithelium and Establishment of a Feeder-Free and Serum-Free Culture System

Cornea
Satoru YamagamiTohru Sakimoto

Abstract

Transplantation of the autologous cultured corneal limbal epithelium and oral mucosal epithelium is a standard technique for ocular surface reconstruction under corneal limbal stem cell deficiency. As an option for bilateral cases, we recommend utilization of autologous conjunctivae for ocular surface reconstruction. Autologous conjunctival epithelium sheet transplantation was effective for bilateral corneal limbal stem cell deficiency without symblepharon or severe keratinization. Moreover, we established a feeder-free and serum-free culture system of the limbal epithelium. This system can be applied for culturing conjunctival epithelia. Autologous cultured conjunctival epithelium transplantation is a practical option for treating bilateral corneal limbal stem cell deficiency.

References

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Apr 27, 2005·Investigative Ophthalmology & Visual Science·Saiko UchidaShiro Amano
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Feb 7, 2006·American Journal of Ophthalmology·Tsutomu InatomiShigeru Kinoshita
Feb 28, 2007·Ophthalmology·Satoru YamagamiShiro Amano
Aug 1, 2009·Investigative Ophthalmology & Visual Science·Leonard P K AngShigeru Kinoshita
Aug 12, 2010·Japanese Journal of Ophthalmology·Tomohiko UsuiShiro Amano
Aug 29, 2013·Stem Cells Translational Medicine·Hideyuki MiyashitaShigeto Shimmura

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