Odontoblast markers and dentine reactions in carious primary molars with and without hypomineralised enamel defects.

International Journal of Paediatric Dentistry
Janita ShahSadna Rajan

Abstract

Wnt/β-Catenin signalling and DMP1 have key roles in tertiary dentinogenesis. To compare the relationship between remaining dentine thickness (RDT), tertiary dentine thickness (TDT), β-catenin and dentine matrix protein 1 (DMP1) in carious second primary molar teeth with normal (SPM) and hypomineralised enamel (HSPM). Extracted carious SPM and HSPM were fixed, sectioned (5 μm) and stained with haematoxylin and eosin or with indirect immunofluorescence for β-catenin and DMP1. Image analysis was performed to determine RDT, TDT, β-catenin and DMP1 intensity in the odontoblast layer and dentine-pulp complex. Carious SPM (n = 11; mean RDT = 1536.1 μm) and HSPM (n = 12; mean RDT = 1179.9 μm) had mean TDT 248.6 μm and 518.1 μm, respectively (P = .02). There were no significant differences in intensity values in the odontoblast layer and dentine-pulp complex for β-catenin and DMP1 for both groups. There was no observable variation in Wnt/β-catenin and DMP1 expression between HSPM and SPM despite a statistically significant twofold increased TDT in HSPM compared with SPM that had similar RDT. Thus, the observed increased TDT in HSPM is more likely due to an earlier onset of repair processes rather than an amplified response to caries.

References

Dec 14, 2007·European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry·H D RoddF M Boissonade
Jun 29, 2010·Journal of Dentistry·Paul R CooperAnthony J Smith
Sep 16, 2011·Journal of Dental Research·X L HanJ Q Feng
Dec 14, 2011·Journal of Endodontics·Ashraf Abd-ElmeguidHarissios Vliagoftis
Jan 6, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Daniel J HunterJill A Helms
Feb 14, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Natalino Lourenço NetoThais M Oliveira
Nov 6, 2015·Mediators of Inflammation·Jean-Christophe FargesPaul R Cooper
Jan 10, 2017·Scientific Reports·Vitor C M NevesPaul T Sharpe
Feb 7, 2018·International Endodontic Journal·W L O da RosaA F da Silva
Mar 7, 2019·Methods in Molecular Biology·R C BabbP T Sharpe
Aug 11, 2019·Nature Communications·Jemma Victoria WalkerBing Hu
Mar 12, 2020·Journal of Dental Research·L K ZauggP T Sharpe

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.