Odorant-binding proteins expression patterns in recently diverged species of Anastrepha fruit flies

Scientific Reports
Emeline Boni CampaniniReinaldo A de Brito

Abstract

We studied two species of closely related South American fruit flies, Anastrepha fraterculus and Anastrepha obliqua which, despite being able to interbreed, still show some ecological and reproductive differences. Because part of these differences, such as host and mate preferences, may be related to olfactory perception, we focused our investigation on the differential expression of Odorant-binding protein (OBP) gene family, which participate in initial steps of the olfactory signal transduction cascade. We investigated patterns of expression of eight OBP genes by qPCR in male and female head tissues of both species. The expression patterns of these OBPs suggest that some OBP genes are more likely involved with the location of food resources, while others seem to be associated with mate and pheromone perception. Furthermore, the expression patterns obtained at different reproductive stages indicate that OBP expression levels changed significantly after mating in males and females of both species. All eight OBP genes analyzed here showed significant levels of differential expression between A. fraterculus and A. obliqua, suggesting that they may hold important roles in their olfactory perception differences, and consequently, m...Continue Reading

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Citations

Feb 26, 2019·Royal Society Open Science·Peng-Cheng Liu, De-Jun Hao
May 6, 2021·Insects·Francesca ScolariLucie Vaníčková
Mar 15, 2019·Journal of Agricultural and Food Chemistry·Bowen TangChunni Zhang
Aug 25, 2021·Genome Biology and Evolution· MrinaliniNalini Puniamoorthy

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Methods Mentioned

BETA
RNA-seq
PCR
electrophoresis

Software Mentioned

GraphPad
SAMtools
Trinity
eXpress
Primer
Bowtie
edgeR
MAFFT
Prism
Rad CFX Manager [UNK]

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