PMID: 15353077Sep 9, 2004Paper

Oestradiol, cyclodextrin-encapsulated 17beta-oestradiol and the oestradiol solubilizer 2-hydroxypropyl-beta-cyclodextrin all impair preimplantation mouse embryo development

Reproductive Biomedicine Online
L KaragencD K Gardner

Abstract

The aim of this study was to examine the effects of 2-hydroxypropyl-beta-cyclodextrin (HbetaC) used as a solubilizer for oestradiol, 17beta-oestradiol (ethanol soluble) and HbetaC-encapsulated-17beta-oestradiol on mouse embryo development in vitro. HbetaC had no effect on day 3 development. In contrast, blastocyst development and blastocyst cell number were significantly reduced in the presence of 10(-4) mol/l solubilizer equivalent, but not at lower concentrations. The proportion of compacted embryos was significantly reduced with 10(-4) mol/l 17beta-oestradiol. No blastocysts were formed at 10(-4) mol/l concentration of 17beta-oestradiol, although the rate of blastocyst formation did not differ at lower concentrations. Blastocyst cell number was significantly decreased compared with controls at 10(-5) mol/l 17beta-oestradiol. The dose-response using HbetaC-encapsulated-17beta-oestradiol revealed that at 17beta-oestradiol concentrations of 10(-4) and 10(-5) mol/l, blastocyst development was significantly reduced. Blastocyst cell number was significantly reduced compared with controls for all concentrations of HbetaC-encapsulated-17beta-oestradiol. Exposure of embryos to 17beta-oestradiol (10(-4) mol/l) reduced blastocyst devel...Continue Reading

References

Nov 1, 1992·Journal of Reproduction and Fertility·G Ertzeid, R Storeng
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·Q Hou, J Gorski
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·Q HouJ Gorski
Dec 22, 1999·Molecular Reproduction and Development·C YingY Yang
Feb 7, 2001·Human Reproduction·G Ertzeid, R Storeng
May 2, 2001·Human Reproduction·M T LangleyK J Doody
Jun 2, 2001·Human Reproduction·I Van der Auwera, T D'Hooghe
Jun 4, 2002·Human Reproduction·Ingrid Van der AuweraThomas M D'Hooghe

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Citations

May 24, 2015·Workplace Health & Safety·Judith E ArnetzLynnette Essenmacher
Jul 31, 2013·New Solutions : a Journal of Environmental and Occupational Health Policy : NS·Jane A Lipscomb, Mazen El Ghaziri

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