Oestrogen-activated autophagy has a negative effect on the anti-osteoclastogenic function of oestrogen

Cell Proliferation
Liang ChengDenghui Xie

Abstract

Oestrogen is known to inhibit osteoclastogenesis, and numerous studies have identified it as an autophagic activator. To date, the role of oestrogen in the autophagy of osteoclast precursors (OCPs) during osteoclastogenesis remains unclear. This study aimed to determine the effect of autophagy regulated by the biologically active form of oestrogen (17β-estradiol) on osteoclastogenesis. After treatment with 17β-estradiol in OCPs (from bone marrow-derived macrophages, BMMs) and ovariectomy (OVX) mice, we measured the effect of 17β-estradiol on the autophagy of OCPs in vitro and in vivo. In addition, we studied the role of autophagy in the OCP proliferation, osteoclast differentiation and bone loss regulated by 17β-estradiol using autophagic inhibitor or knock-down of autophagic genes. The results showed that direct administration of 17β-estradiol enhanced the autophagic response of OCPs. Interestingly, 17β-estradiol inhibited the stimulatory effect of receptor activator of nuclear factor-κB ligand (RANKL) on the autophagy and osteoclastogenesis of OCPs. Moreover, 17β-estradiol inhibited the downstream signalling of RANKL. Autophagic suppression by pharmacological inhibitors or gene silencing enhanced the inhibitory effect of 17β-...Continue Reading

References

Feb 8, 1992·Lancet·D M Reid, D W Purdie
Aug 24, 1991·BMJ : British Medical Journal·M R LawT W Meade
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T Takano-Yamamoto, G A Rodan
Dec 1, 1982·The Journal of Clinical Endocrinology and Metabolism·C ChristiansenI B Transbøl
Aug 22, 2001·JAMA : the Journal of the American Medical Association·D T VillarealW M Kohrt
Aug 21, 2002·JAMA : the Journal of the American Medical Association·Heidi D NelsonJanet D Allan
Dec 28, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiaojun WuJay M McDonald
Jan 13, 2005·The Journal of Biological Chemistry·Verónica García PalaciosHarry C Blair
Oct 3, 2007·Cell Metabolism·Deborah V Novack
Oct 6, 2009·Biochemical and Biophysical Research Communications·Xiumin LuLin Chen
Apr 2, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeongim HaHong-Hee Kim
Nov 8, 2011·Developmental Cell·Carl J DeSelmHerbert W Virgin
Mar 3, 2012·American Journal of Respiratory and Critical Care Medicine·Tim LahmIrina Petrache
May 19, 2012·Trends in Endocrinology and Metabolism : TEM·Sundeep KhoslaDavid G Monroe
Sep 15, 2012·Annals of the Rheumatic Diseases·Neng-Yu LinJörg H W Distler
Feb 20, 2013·Nature Reviews. Endocrinology·Serge RozenbergCaroline Antoine
Oct 24, 2013·Journal of Cellular Biochemistry·Fangjing ChenAimin Chen
Dec 10, 2013·The Journal of Clinical Investigation·Yan XiuBrendan F Boyce
Jul 1, 2014·Archives of Biochemistry and Biophysics·Alfredo CapparielloAnna Teti
Jul 11, 2014·Trends in Molecular Medicine·Julia F Charles, Antonios O Aliprantis
Aug 29, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rui-Fang LiYi-Fang Zhao
Jun 27, 2015·Annals of the Rheumatic Diseases·Neng-Yu LinJörg Hw Distler
Jun 30, 2015·BioMed Research International·Qi XiongWei Ge
Mar 25, 2016·Scientific Reports·Pierangela TottaFilippo Acconcia
Apr 14, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Nabil F Saba, Stuart J Wong
Jul 27, 2017·Scientific Reports·Carmen StreicherReinhold G Erben
Aug 29, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lan ZhangJake Chen
Feb 13, 2018·Nature Communications·Sergey KarakashevRugang Zhang
Dec 24, 2018·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Shubhangi GavaliM Ikram Khatkhatay

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Citations

Jan 17, 2021·Journal of Cellular Physiology·Yi-Fan GuoXiang-Hang Luo
Dec 29, 2020·Research in Veterinary Science·Naixi YangRuili Zhang
Jan 8, 2021·Journal of Molecular Histology·Rinaldo Florencio-SilvaPaulo Sérgio Cerri
Jun 15, 2020·Ageing Research Reviews·Xu LiLing Qin

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Methods Mentioned

BETA
PCR
fluorescence microscopy
immunoprecipitation
flow cytometry

Software Mentioned

SPSS
Pro Plus ( IPP
Image

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