Oestrogen imprinting causes nuclear changes in epithelial cells and overall inhibition of gene transcription and protein synthesis in rat ventral prostate

International Journal of Andrology
Taize M AugustoHernandes F Carvalho

Abstract

Oestrogen exposure during the early post-natal period affects male growth, physiology, and susceptibility to disease in adult life. The prostate gland is susceptible to this oestrogen imprinting, showing a reduced expression of the androgen receptor and inability to respond to androgen stimulus. In this context, we decided to study key signalling regulators of ventral prostate (VP) functioning after early postnatal exposure to high-dose oestrogen. Our results showed a decrease of mTOR phosphorylation and its direct downstream target 4EBP. It is known that mTOR-induced signalling is a pivotal pathway of cell metabolism, which is able to control gene transcription and protein synthesis. We then decided to investigate other indicators of a reduced metabolism in the oestrogenized prostate, and found that the luminal epithelial cells were shorter, less polarized and had smaller nuclei containing more compacted chromatin, suggesting that a general mechanism of regulating gene expression and protein synthesis could be installed in the epithelium of the oestrogenized VP. To evaluate this idea, we analysed nucleolar morphology, and measured the amount of ribosomes and the level of methylation of the 45S ribosomal RNA promoter region. Th...Continue Reading

References

Aug 1, 1991·Biology of Reproduction·N HayashiG R Cunha
Jul 10, 1982·Nucleic Acids Research·L Kunnath, J Locker
Oct 15, 1982·International Journal of Cancer. Journal International Du Cancer·K Kano-Tanaka, T Tanaka
Jan 1, 1994·The Prostate·R SanttiJ A McLachlan
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·A Y LiuG van den Engh
Aug 31, 2000·Endocrinology·R A JarredG P Risbridger
Apr 12, 2002·Trends in Endocrinology and Metabolism : TEM·Renea A JarredGail P Risbridger
Nov 26, 2002·Endocrinology·Joseph John BiancoGail Petuna Risbridger
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christopher C OakesBernard Robaire
Apr 17, 2003·Endocrinology·Jason P GilleranGail S Prins
Jun 18, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M García-FlórezH F Carvalho
Feb 14, 2006·Cell·Stephan WullschlegerMichael N Hall
Jan 31, 2007·Annals of the New York Academy of Sciences·Gail S PrinsYongbing Pu
Feb 27, 2007·Cell·Bradley E BernsteinEric S Lander
May 25, 2007·Journal of Andrology·Eliane AntonioliHernandes F Carvalho
Sep 12, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Alexandre Bruni-Cardoso, Hernandes F Carvalho
Jun 26, 2008·Reproduction, Fertility, and Development·Daniela C C GerardinOduvaldo C M Pereira
Jul 12, 2008·Annual Review of Cell and Developmental Biology·Brian McStay, Ingrid Grummt

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Citations

Sep 6, 2011·Histochemistry and Cell Biology·Taize M AugustoHernandes F Carvalho
Mar 1, 2012·Histochemistry and Cell Biology·Stefan Hübner, Athina Efthymiadis
Feb 17, 2017·Cell Biology International·Guilherme O BarbosaTaize M Augusto

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