Oestrogen promotes lipids transportation through oestrogen receptor α in hepatic steatosis of geese in vitro.

Journal of Animal Physiology and Animal Nutrition
Yan DengJiwen Wang

Abstract

Evidence has shown that oestrogen suppresses lipids deposition in the liver of mammals. However, the molecular mechanism of oestrogen action in hepatic steatosis of geese liver has yet to be determined. This study aimed to investigate the effect of oestrogen on lipid homeostasis at different states of geese hepatocytes in vitro. The results showed that an in vitro model of hepatic steatosis was induced by 1.5 mM sodium oleate via detecting the viability of hepatocytes and content of lipids. When the normal hepatocytes were administrated with different concentrations of oestrogen (E2 ), the expression levels of diacylglycerol acyltransferase 2 (DGAT2), microsomal triglyceride transfer protein (MTTP) and oestrogen receptors (ERs, alpha and beta) were up-regulated only at high concentrations of E2 , whereas the lipid content was not a significant difference. In goose hepatocytes of hepatic steatosis, however, the expression levels of MTTP, apolipoprotein B (apoB) and ERα/β significantly increased at 10-7 or 10-6  M E2 . Meanwhile, the lipids content significantly increased at 10-9 and 10-8  M E2 and decreased at 80 µM E2 . Further heatmap analysis showed that ERα was clustered with apoB and MTTP in either normal hepatocytes or tha...Continue Reading

References

May 29, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J MourotD Hermier
Mar 5, 2003·BioTechniques·A I SaeedJ Quackenbush
Apr 8, 2004·Current Atherosclerosis Reports·Jean-Francois LouetFranck Mauvais-Jarvis
May 15, 2008·Journal of Animal Physiology and Animal Nutrition·P NguyenH Dumon
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Aug 14, 2008·American Journal of Physiology. Endocrinology and Metabolism·Galyna BryzgalovaKarin Dahlman-Wright
Feb 12, 2009·Journal of Gastroenterology and Hepatology·Matteo RicchiPaola Loria
Jul 6, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Anna Foryst-Ludwig, Ulrich Kintscher
Dec 15, 2010·Molecular and Cellular Biochemistry·Zhixiong PanBo Kang
Sep 13, 2011·Cell Metabolism·Rodrigo P A Barros, Jan-Åke Gustafsson
Oct 24, 2012·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Eduardo Hideo GilglioniClairce Luzia Salgueiro-Pagadigorria
Nov 28, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Song-iee HanJunn Yanagisawa
Mar 21, 2015·The Journal of Biological Chemistry·Virginia PardoÁngela M Valverde
Mar 23, 2017·General and Comparative Endocrinology·Xue-Fan CuiGuang-Li Li
Dec 11, 2017·Advances in Experimental Medicine and Biology·Brian T PalmisanoJohn M Stafford
Feb 12, 2019·Journal of Surgical Oncology·Kirsi SanttiCarl Blomqvist

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Biology of Lipids

Lipids are essential in many biological functions, including membrane structure, energy storage, and cell signaling. Find the latest research on the cell biology of lipids here.

© 2021 Meta ULC. All rights reserved