Oestrogen receptor α mediates 17β-estradiol enhancement of ovarian cancer cell motility through up-regulation of survivin expression.

Archives of Gynecology and Obstetrics
Jing ZhuYou-Ji Feng

Abstract

To determine the role of oestrogen receptor α (ERα) in the regulation of survivin expression by 17β-estradiol (E(2)) in ovarian cancer cells and to evaluate the mechanism of E(2) action on ovarian cancer cell migration. We performed RT-PCR and Western blot analysis to assess the expression of ERα in the ovarian cancer cell lines NIH:OVCAR-3 and SKOV-3. Full-length ERα cDNA was reintroduced into SKOV-3 cells through stable transfection. After treatment with E(2), with or without pre-incubation of anti-oestrogen compound ICI 182780, RT-PCR and Western blot analysis were performed to detect survivin expression at the mRNA and protein levels. RNA interference (RNAi) was used to inhibit the expression of survivin in SKOV-3 cells. Wound healing-induced migration and Matrigel invasion experiments were performed to determine the motility of ovarian cancer cells. RT-PCR and gelatin zymography were used to detect the expression and activity of MMP-9 in SKOV-3 cells. A stably transfected clone with over-expression of ERα, SKOV-α, was isolated. Exogenous or endogenous expression of ERα in SKOV-3 or NIH:OVCAR-3 cells resulted in a significant up-regulation of survivin in the presence of E(2). Pre-treatment with ICI 182780 attenuated the up-...Continue Reading

References

Jan 1, 1995·Journal of Cellular Biochemistry. Supplement·R E Scully
Mar 29, 2000·CA: a Cancer Journal for Clinicians·R T GreenleeP A Wingo
Mar 23, 2001·JAMA : the Journal of the American Medical Association·C RodriguezM J Thun
Jun 1, 2001·The Journal of Steroid Biochemistry and Molecular Biology·H RochefortM Garcia
Nov 21, 2001·The Journal of Biological Chemistry·William H HoffmanMaureen Murphy
Oct 3, 2002·Nature Reviews. Genetics·Michael T McManus, Phillip A Sharp
Nov 21, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Tetsuhisa YamamotoNobuhiko Tanigawa
Oct 2, 2003·JAMA : the Journal of the American Medical Association·Garnet L AndersonUNKNOWN Women's Health Initiative Investigators
Nov 24, 2004·Veterinary Pathology·M E Johnson, E W Howerth
Apr 20, 2006·Cancer Letters·N K SahP S Bisen
Jan 24, 2007·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Mar 8, 2007·Carcinogenesis·Marzia PennatiNadia Zaffaroni
Sep 13, 2007·Journal of the National Cancer Institute·Jessica D AlbanoMichael J Thun
Jan 4, 2008·Pathology, Research and Practice·Pauline AthanassiadouEfstratios Patsouris
Jun 30, 2009·Cancer Treatment Reviews·Bríd M RyanMichael J Duffy
Aug 21, 2010·Applied Immunohistochemistry & Molecular Morphology : AIMM·Xueya QianLinxia Li
Oct 1, 2010·Molecular Endocrinology·Angel Matias SanchezTommaso Simoncini

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Citations

Oct 10, 2012·Endocrine-related Cancer·Francesmary ModugnoSteffi Oesterreich
Dec 18, 2013·Steroids·Vinciane Saint-Criq, Brian J Harvey
Jan 2, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Junjun QiuKeqin Hua
Aug 13, 2015·Archives of Gynecology and Obstetrics·Shi-tao ZhangXiao-ping Zhang
Jul 8, 2015·British Journal of Cancer·C M NagleUNKNOWN Ovarian Cancer Association Consortium
Oct 5, 2018·Hormones & Cancer·Waleeporn KaewlertRaynoo Thanan

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