Oestrogen receptor subtype-specific repression of calpain expression and calpain enzymatic activity in neuronal cells--implications for neuroprotection against Ca-mediated excitotoxicity

Journal of Neurochemistry
Martin GamerdingerChristian Behl

Abstract

Calpains represent a superfamily of Ca2+-activated cysteine-proteases, which are important mediators of apoptosis and necrosis. In the brain, m-calpain and micro-calpain, the two ubiquitous calpain-isoforms, are strongly activated in neurones after an excitotoxic Ca2+ influx occurring, for example, during cerebral ischemia. Because oestrogen and its receptors (ERalpha/ERbeta) can exert neuroprotective activity, we investigated their influence on expression of calpains and their endogenous inhibitor, calpastatin. We found that ectopic expression of ERalpha in human neuroblastoma SK-N-MC cells led to a ligand-independent constitutive down-regulation of m-calpain accompanied by an up-regulation of micro-calpain expression. Up-regulation of micro-calpain was reversed in the presence of oestrogen, which, in turn, could be blocked by co-treatment with the oestrogen-receptor antagonist ICI 182,780. Expression of calpastatin was not altered, either in the absence or in the presence of oestrogen. Additional studies revealed that ERalpha-expressing cells exhibited decreased calpain enzymatic activity and increased survival when cells were exposed to the Ca2+ ionophore, ionomycin. Since all investigated effects could be observed exclusive...Continue Reading

References

Jul 1, 1991·Physiological Reviews·D E Croall, G N DeMartino
Jun 30, 1988·Biochemical and Biophysical Research Communications·T TsujinakaT Mori
Dec 15, 1994·Annals of the New York Academy of Sciences·R A NixonM Beermann
Mar 1, 1994·Stroke; a Journal of Cerebral Circulation·S C HongK S Lee
Dec 1, 1995·Annals of Neurology·D E Bredesen
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·G G KuiperJ A Gustafsson
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·K K WangP Yuen
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·A N MurphyG Fiskum
Feb 7, 1998·The Biochemical Journal·H SorimachiK Suzuki
Nov 4, 1998·Frontiers in Neuroendocrinology·G G KuiperJ A Gustafsson
Nov 11, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D B DubalP M Wise
May 5, 1999·Neurology·R Saunders-PullmanS Bressman
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·B Moosmann, C Behl
Jun 30, 2000·Human Reproduction Update·M Beato, J Klug
Oct 24, 2000·Oncogene·K PetterssonJ A Gustafsson
Feb 13, 2001·International Journal of Experimental Pathology·P W Vanderklish, B A Bahr
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·D B DubalP M Wise
Jun 19, 2001·The Journal of Biological Chemistry·M ChenR A Gottlieb
Jan 22, 2002·Reproduction, Fertility, and Development·T Ratajczak
Feb 19, 2002·The Journal of Steroid Biochemistry and Molecular Biology·J F Robertson
May 10, 2002·The Journal of Biological Chemistry·Shirley Gil-ParradoWerner Machleidt
Jun 4, 2002·Nature Reviews. Neuroscience·Christian Behl
Jul 9, 2002·EMBO Reports·Popi Syntichaki, Nektarios Tavernarakis
Jul 20, 2002·Neuropharmacology·Hideyuki SawadaShun Shimohama
Jul 26, 2002·The Journal of Biological Chemistry·Jürgen ZschockeChristian Behl
Aug 2, 2002·Biochemical and Biophysical Research Communications·W Rachel DuanJ Larry Jameson
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jon Nilsen, Roberta Diaz Brinton
Mar 26, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Christian Behl
Apr 23, 2003·Brain Research·Pratima SurNaren Lal Banik
Apr 25, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Jürgen ZschockeChristian Behl
Jul 5, 2003·Physiological Reviews·Darrell E GollJinyang Cong
Sep 5, 2003·Nucleic Acids Research·Lihua DingQinong Ye
Jan 10, 2004·Endocrine-related Cancer·K M DobrzyckaS Oesterreich
Jan 14, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amy M FowlerElaine T Alarid

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Citations

May 15, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lawrence D BrewerEric M Blalock
May 12, 2007·Protein Expression and Purification·Vinay Kumar SinghZongchao Jia
Feb 21, 2009·The EMBO Journal·Martin GamerdingerChristian Behl
Nov 11, 2009·Journal of Receptor and Signal Transduction Research·Volodymyr RybalchenkoPeter Koulen
Mar 25, 2008·Neuropharmacology·Martin GamerdingerChristian Behl
Apr 28, 2012·Neuroendocrinology·James W SimpkinsAnne M Etgen
May 22, 2009·Journal of Neurochemistry·Monica AvernaEdon Melloni
Apr 22, 2015·Bioorganicheskaia khimiia·L A LysenkoN N Nemova
May 1, 2018·Frontiers in Bioengineering and Biotechnology·Margarita T AngelovaJean-Yves Roignant
Sep 1, 2013·Bioorganicheskaia khimiia·L A LysenkoN N Nemova
Jul 8, 2008·Taiwanese Journal of Obstetrics & Gynecology·Kung-Hao HsuYao-Yuan Hsieh

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