PMID: 6401794Jan 1, 1983Paper

Oestrogen secretion by in-vitro perfused testes: species comparison and factors affecting short-term secretion

The Journal of Endocrinology
D L ThompsonB L Lasley

Abstract

Testes from mature rats, rabbits, hamsters, guinea-pigs and dogs were perfused in vitro with added gonadotrophins to study the qualitative and quantitative aspects of oestrogen secretion by these species. After separation by high performance liquid chromatography (HPLC), the major oestrogen secreted by the testes of all five species was oestradiol-17 beta; lesser amounts of oestrone were also detected for all species. Secretion rates of oestradiol and testosterone were determined for one testis from each of six animals of each species after purification by HPLC. Oestradiol and testosterone secretion varied (P less than 0.05) among species on both per testis and per gram of testis bases. The rabbit, which was a high secretor of oestradiol, was used in subsequent experiments to study the factors which affect short-term oestradiol secretion by perfused testes. Luteinizing hormone (NIAMDD-LH-S21) at 100 ng/ml significantly stimulated oestradiol secretion by 2.9-fold and increased testosterone secretion by 155-fold. Follicle-stimulating hormone (NIH-FSH-S11) had no significant effect on secretion of either steroid. In a time-course study in which LH stimulation was started after 45 min of control perfusion, the rates of increase in ...Continue Reading

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