PMID: 11929845Apr 4, 2002Paper

Oestrogenic repression of human coagulation factor VII expression mediated through an oestrogen response element sequence motif in the promoter region

Human Molecular Genetics
Rosa Di BitondoPeter R Winship

Abstract

Reporter gene analysis of two regions of the human factor VII (FVII) gene promoter (residues -658 to -1 and -348 to -1, where +1 is the start site of translation) in the mammalian liver-derived cell line HepG2 showed reduced transcriptional activity in the presence of oestrogenic factors. This effect was independent of promoter polymorphic haplotype. Similar analysis using a smaller region of the promoter spanning residues -187 to -1 failed to show any evidence of oestrogenic suppression. Electrophoretic mobility shift assays and supershift assays using recombinant oestrogen receptor alpha and anti-oestrogen receptor antibody localized the sequence motif to which oestrogen receptor was binding to residues -225 to -212 of the FVII promoter. The lack of oestrogenic suppression in a reporter gene construct spanning residues -658 to -1 modified to abolish oestrogen receptor binding at this site, confirmed the functional significance of this motif. Although superficially similar to the classical oestrogen response element (ORE), comprising two half sites separated by three spacer nucleotides, the FVII ORE represents an alternative type of ORE in which the two half sites are separated by just two spacer nucleotides. EMSAs indicated t...Continue Reading

Citations

Aug 26, 2010·Nanomedicine·Joaquin SanchisMaría J Vicent
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·J A CarewK A Bauer
Oct 25, 2006·Journal of Thrombosis and Haemostasis : JTH·A P ReinerD A Nickerson
Oct 26, 2006·British Journal of Haematology·Adrian J HallPeter R Winship
Jun 8, 2010·Journal of Thrombosis and Haemostasis : JTH·A C A CleurenB J M Van Vlijmen
Dec 11, 2014·World Journal of Clinical Oncology·Shiro Koizume, Yohei Miyagi
Sep 24, 2015·Biomarkers in Cancer·Shiro Koizume, Yohei Miyagi
Nov 11, 2016·Experimental Animals·Yanira Franco-Murillo, Ruth Jaimez

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