Off-line coupling of non-aqueous reversed-phase and silver ion high-performance liquid chromatography-mass spectrometry for the characterization of rice oil triacylglycerol positional isomers

Journal of Chromatography. a
P DugoL Mondello

Abstract

The determination of the triacylglycerol (TAG) profile in real world matrices is rather difficult as these compounds present a complex composition and are characterized by similar physico-chemical properties. This investigation is based on the high-performance liquid chromatography (HPLC) multidimensional determination of the TAG profile in terms of TAG species and positional isomers in a rice oil sample. The off-line bi-dimensional system was attained through the coupling of non-aqueous reversed-phase HPLC and silver ion (Ag)-HPLC. The primary column eluate was fractionated and the fractions of interest were then injected onto the secondary column, allowing the separation of several TAG positional isomers, unresolved in the first dimension. Peak assignment was carried out by combining retention data with atmospheric pressure chemical ionisation (APCI) MS spectra information. The fatty acid distribution along the glycerol backbone, determined by Ag-HPLC, was confirmed through diglyceride ion ratios derived from APCI-MS analysis. Method validation, where both precision and accuracy were measured, was carried out in preliminary applications on standard compounds. The analytical results obtained show that rice oil TAGs follow a di...Continue Reading

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Citations

Jun 30, 2011·Analytical and Bioanalytical Chemistry·Francesco SaliuMaria Perla Colombini
Oct 22, 2010·Lipids·Pedro AraujoBjørn Grung
Nov 8, 2011·Journal of Agricultural and Food Chemistry·Chiara FanaliLuigi Mondello
Dec 12, 2012·Journal of Agricultural and Food Chemistry·Xiao-Qiang ZouXing-Guo Wang
Apr 1, 2014·Analytical and Bioanalytical Chemistry·Su Jung KimHyun Ju Yoo
Mar 25, 2011·Immunopharmacology and Immunotoxicology·Biagina ChiofaloLuigi Mondello
Apr 3, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qin YangGuowang Xu
Mar 27, 2012·Journal of Chromatography. a·Pavel Jandera
Nov 26, 2008·Journal of Chromatography. a·Boryana Nikolova-Damyanova
Jun 26, 2007·Rapid Communications in Mass Spectrometry : RCM·Heidi LeskinenHeikki Kallio
Feb 29, 2008·Journal of Separation Science·Yanming LiuXinmiao Liang
Sep 15, 2006·Journal of Separation Science·Pavel Jandera
Oct 18, 2011·Journal of Separation Science·Christian HellmuthBerthold Koletzko
Feb 14, 2006·Journal of Chromatography. a·Sheng-Suan Cai, Jack A Syage
Oct 26, 2005·Journal of Chromatography. a·Josef CvackaIrena Valterová
Jun 16, 2015·Journal of Agricultural and Food Chemistry·Mikhail VyssotskiD Bradley G Williams
Oct 25, 2014·Applied Biochemistry and Biotechnology·Ibrahim O Mohamed
May 18, 2016·Analytical Chemistry·Y-L Elaine WongT-W Dominic Chan
Jun 19, 2016·Analytical Biochemistry·Tomáš ŘezankaKarel Sigler
Feb 21, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jae Won LeeTakeshi Bamba

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