OGP functionalized phenylalanine-based poly(ester urea) for enhancing osteoinductive potential of human mesenchymal stem cells

Biomacromolecules
Gina M PolicastroMatthew L Becker

Abstract

Amino acid-based poly(ester urea)s (PEU) are high modulus, resorbable polymers with many potential uses, including the surgical repair of bone defects. In vitro and in vivo studies have previously shown that phenylalanine-based PEUs have nontoxic hydrolytic byproducts and tunable degradation times. Phenylalanine PEUs (poly(1-PHE-6)) have been further modified by tethering osteogenic growth peptide (OGP) to tyrosine-based monomer subunits. These OGP-tethered PEUs have been fabricated into porous scaffolds and cultured in vitro to examine their effect on differentiation of human mesenchymal stem cells (hMSCs) toward the osteogenic lineage. The influence of tethered OGP on the hMSC proliferation and differentiation profile was measured using immunohistochemistry, biochemistry, and quantitative real time polymerase chain reaction (qRT-PCR). In vitro data indicated an enhanced expression of BSP by 130-160% for hMSCs on OGP-tethered scaffolds compared to controls. By 4 weeks, there was a significant drop (60-85% decrease) in BSP expression on OGP-functionalized scaffolds, which is characteristic of osteogenic differentiation. ALP and OSC expression was significantly enhanced for OGP-functionalized scaffolds by week 4, with values rea...Continue Reading

References

Jun 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L D QuarlesR J Wenstrup
Dec 1, 1992·Journal of Biomedical Materials Research·Y MatsusueY Ikada
Mar 1, 1992·Nutrition Reviews·R T Franceschi
Jan 1, 1994·Journal of Biomaterials Science. Polymer Edition·C MigliaresiD Cohn
Feb 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K L YaoJ Sodek
Apr 2, 1999·Science·M F PittengerD R Marshak
Oct 14, 2000·Annual Review of Cell and Developmental Biology·E D Rosen, B M Spiegelman
Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
Feb 7, 2006·Biomaterials·Chunmei LiDavid L Kaplan
May 1, 2007·Biomaterials·Kai Guo, Chih-Chang Chu
Feb 28, 2008·Journal of Periodontal Research·Y Ogata
Nov 27, 2008·Cell and Tissue Research·Xiaobing Fu, Haihong Li
Aug 5, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Manisha C YadavJosé Luis Millán
Dec 1, 2012·Stem Cells Translational Medicine·Scott MaxsonMichelle A Leroux
Jan 1, 1998·Journal of Cellular Biochemistry·Jane E Aubin

❮ Previous
Next ❯

Citations

Sep 24, 2015·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Gina M Policastro, Matthew L Becker
Jul 5, 2016·Macromolecules·Andrea S CarliniNathan C Gianneschi
Oct 26, 2016·Journal of the American Chemical Society·Guoqing PanHuilin Yang
Nov 24, 2016·International Journal of Molecular Sciences·Suzane C PigossiRaquel M Scarel-Caminaga
Mar 10, 2017·Journal of Biomaterials Science. Polymer Edition·Zhixiang CuiWenzhe Chen
Sep 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Alex M JordanLaShanda T J Korley
Sep 28, 2018·Journal of Biomedical Materials Research. Part a·Ju LiuKaiyong Cai
Mar 7, 2021·Annals of Biomedical Engineering·Bharti BishtManash K Paul
Sep 14, 2015·ACS Biomaterials Science & Engineering·Yaohua GaoMatthew L Becker
Jan 23, 2020·Accounts of Chemical Research·Cassandra E CallmannNathan C Gianneschi
Jan 28, 2020·Biomacromolecules·Alexandra K AbelMatthew L Becker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.