Oil body bound oleosin-rhFGF9 fusion protein expressed in safflower (Carthamus tinctorius L.) stimulates hair growth and wound healing in mice

BMC Biotechnology
Jingbo CaiHaiyan Li

Abstract

Fibroblast growth factor 9 (FGF9) is a heparin-binding growth factor, secreted by both mesothelial and epithelial cells, which participates in hair follicle regeneration, wound healing, and bone development. A suitable source of recombinant human FGF9 (rhFGF9) is needed for research into potential clinical applications. We present that expression of oleosin-rhFGF9 fusion protein in safflower (Carthamus tinctorius L.) seeds stimulates hair growth and wound healing. The oleosin-rhFGF9 expressed in safflower seeds, in which it localizes to the surface of oil bodies. The expression of oleosin-rhFGF9 was confirmed by polyacrylamide gel electrophoresis and western blotting. According to BCA and Enzyme-linked immunosorbent assay (ELISA) assay, the results show that the expression level of oleosin-rhFGF9 was 0.14% of oil body protein. The oil body bound oleosin-rhFGF9 showed mitogenic activity towards NIH3T3 cells in a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. The efficacy of oil body bound oleosin-rhFGF9 in promoting hair growth and wound healing was investigated in C57BL/6 mice. In a hair regeneration experiment, 50 μg/μl oil body bound oleosin-rhFGF9 was applied to the dorsal skin of mice in the resting phase of the h...Continue Reading

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Citations

Dec 6, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zhuoyue ChenFulin Chen
May 1, 2021·Vaccines·Jennifer Schwestka, Eva Stoger
Jul 3, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Saeed El-AshramAbeer El Wakil

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Methods Mentioned

BETA
transgenic
PCR
ELISA
electrophoresis
protein assay

Software Mentioned

GraphPad Prism
ImageJ
GraphPad
Genewiz

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