"Oil-soluble" reversed lipid nanoparticles for oral insulin delivery.

Journal of Nanobiotechnology
Tao WangDongqin Quan

Abstract

In this study, we aimed to design a novel oral insulin delivery system, named "oil-soluble" reversed lipid nanoparticles (ORLN), in which a hydrophilic insulin molecule is encapsulated by a phospholipid (PC) shell and dissolved in oil to prevent the enzymatic degradation of insulin. ORLN was characterized by transmission electron microscopy and dynamic light scattering. In vitro enzymatic stability studies showed higher concentrations of insulin in cells incubated with ORLN-encapsulated insulin than in those incubated with free insulin solution in artificial intestinal fluid (pH 6.5). The protective effect of ORLN was attributed to its special release behavior and the formulation of the PC shell and oil barrier. Furthermore, an in vivo oral efficacy study confirmed that blood glucose levels were markedly decreased after ORLN administration in both healthy and diabetic mice. In vivo pharmacokinetic results showed that the bioavailability of ORLN-conjugated insulin was approximately 28.7% relative to that of the group subcutaneously administered with an aqueous solution of insulin, indicating enhanced oral absorption. In summary, the ORLN system developed here shows promise as a nanocarrier for improving the oral absorption of in...Continue Reading

References

Nov 25, 2005·Current Drug Delivery·Dmitry MalkovIsabel Gomez-Orellana
Dec 5, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Christiane DamgéNathalie Ubrich
Dec 25, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Abdallah MakhlofHirofumi Takeuchi
Apr 17, 2012·International Journal of Pharmaceutics·M R Rekha, Chandra P Sharma
Nov 20, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kiran ChaturvediTejraj M Aminabhavi
Feb 23, 2013·International Journal of Pharmaceutics·Jwala RenukuntlaAshim K Mitra
Apr 10, 2013·Journal of Diabetes Science and Technology·Pedro FonteBruno Sarmento
May 31, 2014·Journal of Diabetes Science and Technology·Eric ZijlstraLeona Plum-Mörschel
Jun 28, 2017·Journal of Diabetes Science and Technology·Ehud Arbit, Miriam Kidron
Jun 27, 2018·Proceedings of the National Academy of Sciences of the United States of America·Amrita BanerjeeSamir Mitragotri

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Citations

Jan 9, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yanan WangZhiyu He

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
dynamic light scattering
transmission electron microcopy
ELISA
fluorescence microscopy

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