PMID: 8958447Sep 1, 1996Paper

Okadaic acid as a probe for regulation in vitro of Mg(2+), Ca(2+)-ATPase activity in rat cortical and cerebellar synaptosomal membranes

Cellular Signalling
L ZylinskaL Lachowicz

Abstract

The in vitro effect of okadaic acid on basal phorbol 12-myristate 13-acetate (PMA)-, and cyclic adenosine monophosphate (cAMP)-stimulated Mg(2+)-dependent Ca(2+)-adenosine triphosphatase (ATPase) activity in synaptosomal membranes isolated from rat brain cortex and cerebellum was investigated. The basal activity was enhanced by okadaic acid in both examined regions. This inhibitor differed in the regulation of Mg2+, Ca(2+)-ATPase activity in PMA- and cAMP-incubated membranes. Stimulation by calmodulin (CaM) of basal Mg2+, Ca(2+)-ATPase activity declined in cortex and cerebellum after treatment with okadaic acid. The presence of PMA or cAMP decreases the stimulatory effect of CaM. These results suggest that Mg2+, Ca(2+)-ATPase activity in the rat-brain synaptosomal membrane may be regulated in vitro by dephosphorylation processes.

References

Dec 11, 1992·Brain Research. Molecular Brain Research·W L StahlW R Anderson
Jul 1, 1992·The International Journal of Biochemistry·L Zylińska, L Lachowicz
Jan 1, 1991·Pharmacology & Therapeutics·L MissiaenR Casteels
Jan 1, 1991·Physiological Reviews·E Carafoli
Mar 1, 1990·Trends in Biochemical Sciences·P CohenY Tsukitani
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·G BubA Shrier
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·D S StrausC K Glass
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Zhiyong MaoAndrei Seluanov

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Citations

May 2, 2002·Clinical Biochemistry·Ludmila ZylinskaMiroslaw Soszynski
Dec 10, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K S Rein, J Borrone

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