Oleaginous yeast Yarrowia lipolytica culture with synthetic and food waste-derived volatile fatty acids for lipid production

Biotechnology for Biofuels
Ruiling GaoLei Zheng

Abstract

The sustainability of microbial lipids production from traditional carbon sources, such as glucose or glycerol, is problematic given the high price of raw materials. Considerable efforts have been directed to minimize the cost and find new alternative carbon sources. Volatile fatty acids (VFAs) are especially attractive raw materials, because they can be produced from a variety of organic wastes fermentation. Therefore, the use of volatile fatty acids as carbon sources seems to be a feasible strategy for cost-effective microbial lipid production. Lipid accumulation in Y. lipolytica using synthetic and food waste-derived VFAs as substrates was systematically compared and evaluated in batch cultures. The highest lipid content obtained with acetic, butyric, and propionic acids reached 31.62 ± 0.91, 28.36 ± 0.74, and 28.91 ± 0.66%, respectively. High concentrations of VFA inhibited cell growth in the following order: butyric acid > propionic acid > acetic acid. Within a 30-day experimental period, Y. lipolytica could adapt up to 20 g/L acetic acid, whereas the corresponding concentration of propionic acid and butyric acid were 10 and 5 g/L, respectively. Cultures on a VFA mixture showed that the utilization of different types of VF...Continue Reading

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Citations

Jun 23, 2018·Biotechnology for Biofuels·Young-Kyoung ParkJean-Marc Nicaud
Apr 3, 2019·Frontiers in Microbiology·Adam DobrowolskiAleksandra M Mirończuk
Sep 24, 2019·Biotechnology and Bioengineering·Mercedes LlamasElia Tomás-Pejó
Jan 9, 2021·Bioresource Technology·E Tomás-PejóC González-Fernández
Feb 18, 2021·FEMS Yeast Research·Dmitry Mamaev, Renata Zvyagilskaya
Aug 29, 2021·FEMS Yeast Research·Young-Kyoung ParkPatrick Fickers
Jan 19, 2020·New Biotechnology·Mercedes LlamasCristina González-Fernández

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Methods Mentioned

BETA
fluorescence microscopy
confocal microscopy

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