Oleanolic Acid, a Novel Endothelin A Receptor Antagonist, Alleviated High Glucose-Induced Cardiomyocytes Injury

The American Journal of Chinese Medicine
Dewei WuGuangyuan Song

Abstract

Endothelin-1 (ET-1) and its receptor endothelin A receptor (ET[Formula: see text] have been shown to be upregulated in a high glucose environment, which increase the incidence of diabetes-related heart failure. Our previous study demonstrated that oleanolic acid (OA), a natural compound found in Chinese herbs had ET-1 antagonistic effects. We aimed to verify whether OA could ameliorate diabetes mellitus (DM)-induced injury in cardiomyocytes by reducing the antagonistic effects of the ET-1 pathway. For the induction of high glucose-related injury in cardiomyocytes, neonatal rat ventricular cardiomyocytes (NRVMs) were subjected to culture medium containing 25[Formula: see text]mM of glucose. Natriuretic peptide B (BNP), mitochondrial membrane potential (MMP) and cell surface area were measured to evaluate the severity of NRVMs injury. mRNA expression of ET-1 and ETA was determined using quantitative PCR. Moreover, a Ca[Formula: see text] influx assay was used to evaluate potential ETA antagonistic effects. Molecular docking of OA and ETA was performed using the Sulflex-Dock program. Human induced pluripotent stem cell (iPS-C)-derived cardiomyocytes and real time cell analysis system (RTCA) were used to verify the effect of OA on ...Continue Reading

References

Jul 24, 2001·American Journal of Hypertension·S VermaJ H McNeill
Jun 10, 2008·The New England Journal of Medicine·UNKNOWN Action to Control Cardiovascular Risk in Diabetes Study GroupWilliam T Friedewald
Apr 29, 2011·American Journal of Physiology. Cell Physiology·Sergio de FrutosLaura V Gonzalez Bosc
Feb 5, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Ming LiXuguang Hu
Mar 23, 2018·Molecular Carcinogenesis·Ying XuYufeng Dong
Mar 30, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mlindeli GamedeAndile Khathi

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