Oleic acid and angiotensin II induce a synergistic mitogenic response in vascular smooth muscle cells

Hypertension
G LuB M Egan

Abstract

Oleic acid and angiotensin II (Ang II) are elevated and may interact to accelerate vascular disease in obese hypertensive patients. We studied the effects of oleic acid and Ang II on growth responses of rat aortic smooth muscle cells (VSMCs). Oleic acid (50 micromol/L) raised thymidine incorporation by 50% at 24 hours and cell number by 55% at 6 days (P<.05). Ang II (10(-11) to 10(-6) mol/L) did not significantly increase thymidine incorporation or VSMC number. Combining Ang II and 50 micromol/L oleic acid doubled thymidine incorporation and VSMC number. Losartan, an angiotensin type 1 (AT1) receptor antagonist, blocked the synergistic interaction between Ang II and oleic acid, whereas the AT2 receptor antagonist PD 123319 did not. Protein kinase C inhibition and downregulation, as well as inhibition of extracellular signal-regulated kinase (ERK) activation by PD 98059, eliminated the rise of thymidine incorporation in response to oleic acid and the synergistic interaction with Ang II. However, the response to 10% fetal bovine serum was unaffected. An antisense oligodeoxynucleotide to ERK-1 and ERK-2 reduced ERK protein expression and activation by 83% and 75%, respectively. Antisense prevented the rise of thymidine incorporati...Continue Reading

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Citations

Dec 19, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Kyu Ha HuhYu Seun Kim
Mar 29, 2001·Current Hypertension Reports·B M EganT L Goodfriend
Sep 2, 1999·Prostaglandins, Leukotrienes, and Essential Fatty Acids·B M EganE L Greene
Apr 20, 2001·British Journal of Pharmacology·I Gouni-BertholdA Sachinidis
Nov 13, 2008·The Journal of Clinical Endocrinology and Metabolism·Guillermo E UmpierrezLawrence S Phillips
Sep 30, 2009·Molecular and Cellular Endocrinology·Xiaohong JiangChen-Yu Zhang
Jan 27, 2009·The International Journal of Biochemistry & Cell Biology·Kwan-Lih HsuSeu-Mei Wang
Mar 15, 2008·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Li-Hong FanYong-Xiao Cao
Apr 26, 2005·Journal of Ethnopharmacology·Hong WangBoLi Zhang
Mar 30, 2017·Revista da Associação Médica Brasileira·Phablo AbreuSandro Massao Hirabara
Sep 5, 2002·Journal of Cellular Biochemistry·Jin-Shan ChenSeu-Mei Wang
May 17, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M P StojiljkovicB M Egan
Jan 27, 2021·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Yueting DengChaofu Ke

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