Oleic acid and ergosterol supplementation mitigates oxidative stress in wine strains of Saccharomyces cerevisiae

International Journal of Food Microbiology
Sara LandolfoIlaria Mannazzu

Abstract

During fermentation of high-sugar-containing medium lacking lipid nutrients, wine yeasts undergo oxidative stress and oxidative damage to cell membranes and proteins. Considering that cell membranes are important stress sensors, and that under hypoxic conditions wine yeasts modulate cell membranes composition by incorporating lipids available in the growth medium, in the present work, the effects of lipid nutrition on wine yeast oxidative stress response were evaluated on two strains of Saccharomyces cerevisiae. Biomarkers of oxidative stress, oxidative damage and antioxidant response were evaluated together with viability and acetic acid production during fermentation of a synthetic must lacking lipid nutrients as compared to added oleic acid and ergosterol. The results show that the availability of lipid nutrients causes a significant reduction in the intracellular content of reactive oxygen species and in the oxidative damage to membranes and proteins, as indicated by flow cytometry of cells stained with dihydroethidum (DHE) and propidium iodide (PI) and by Western blot of protein carbonyls. Accordingly, lipid nutrients feeding results in the increase in cell viability and superoxide activity, and the reduction in trehalose ...Continue Reading

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Citations

Sep 21, 2011·Applied Microbiology and Biotechnology·Debra RossouwFlorian F Bauer
May 4, 2012·Applied Microbiology and Biotechnology·Guillaume LemetaisPatrick Gervais
Oct 23, 2012·Journal of Applied Microbiology·J LiuJ Chen
Nov 19, 2013·Environmental Microbiology·Laura TreuStefano Campanaro
Jan 13, 2015·Food Chemistry·Raquel BridiAndrea Versari
Mar 15, 2015·Food Chemistry·Sergey TumanovSilas G Villas-Boas
Oct 14, 2016·World Journal of Microbiology & Biotechnology·Patricia Díaz-HellínAna Briones
Mar 31, 2016·The Biochemical Journal·Nitesh Kumar KhandelwalRajendra Prasad
Jun 8, 2021·Comprehensive Reviews in Food Science and Food Safety·Leticia Casas-GodoyAnne Gschaedler-Mathis
Jul 22, 2021·Biotechnology for Biofuels·Nai-Xin LinXiao-Wei Yu

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