PMID: 9421188Jan 8, 1998Paper

Oleic acid stimulates rapid translocation of cholinephosphate cytidylyltransferase in type II cells

Biochimica Et Biophysica Acta
R M ViscardiJ D Hasday

Abstract

Activity of cholinephosphate cytidylyltransferase, the rate-limiting enzyme in phosphatidylcholine synthesis, increases rapidly in the lung after birth predominantly due to an increase in membrane-associated activity. Although there is strong evidence that enzyme translocation is a major regulatory mechanism in other cells, this mechanism has not been conclusively demonstrated in intact alveolar type II cells. In this study, we show that oleic acid stimulates rapid translocation of cytidylyltransferase activity and protein from cytosol to microsomes in both primary cultured fetal and adult type II cells and MLE12 cells, a cell line derived from murine distal respiratory epithelial cells. Shifts in subcellular distribution occurred within 5 min of exposure to 200 microM oleic acid. The magnitude of the increases in microsomal enzyme activity and immunoreactive protein levels was several-fold greater in d21 fetal cells than adult type II cells. Oleic acid-induced translocation was confirmed in in vitro translocation experiments. After incubating MLE12 cell postmitochondrial supernatants at 37 degrees C with oleic acid and separation of enzyme isoforms on glycerol density gradients, enzyme activity was decreased in gradient fracti...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 15, 1991·Biochimica Et Biophysica Acta·P A WeinholdD A Feldman
Jun 28, 1990·Biochimica Et Biophysica Acta·D A FeldmanP A Weinhold
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G B KalmarR B Cornell
Aug 19, 1987·Biochimica Et Biophysica Acta·M J KreschI Gross
Jan 3, 1986·Biochimica Et Biophysica Acta·E E AeberhardM L Scott
Nov 25, 1983·Journal of Immunological Methods·C Korzeniewski, D M Callewaert
Jan 20, 1995·Biochimica Et Biophysica Acta·J TordayR Slavin
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K A WikenheiserJ A Whitsett
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Mar 29, 2000·The Journal of Biological Chemistry·R K MallampalliS Jackowski
Oct 29, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·C Kim, H C Nielsen
Apr 8, 1999·Progress in Lipid Research·J J Batenburg, H P Haagsman

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