Oleuropein and rutin protect against 6-OHDA-induced neurotoxicity in PC12 cells through modulation of mitochondrial function and unfolded protein response

Interdisciplinary Toxicology
Zubeyir ElmazogluCimen Karasu

Abstract

Parkinson's disease (PD) is a highly prevalent neurodegenerative disorder, often associated with oxidative stress-induced transcriptional changes in dopaminergic neurons. Phenolic antioxidants, oleuropein (OLE) and rutin (RUT) have attracted a great interest due to their potential to counteract oxidative protein aggregation and toxicity. This study aimed at examining the effects of OLE and RUT against 6-OHDA-induced stress response in rat pheochromocytoma cells. When differentiated PC12 cells were exposed to oxidative stress composer 6-OHDA (100 μM, 8 h), a decreased mitochondrial membrane potential (ΔΨm) was observed along with a significant loss of cell viability and apoptotic nuclear changes. Exposure to 6-OHDA resulted in unfolded protein response (UPR) in differentiated PC12 cells as evidenced by an increased level of endoplasmic reticulum (ER)-localized transmembrane signal transducer IRE1α, adaptive response proteins ATF-4 and proapoptotic transcription factor CHOP. OLE or RUT pretreatment (24 h) at low doses (1-50 μM) protected the differentiated PC12 cells from 6-OHDA-induced cytotoxicity as assessed by increased viability, improved ΔΨm and inhibited apoptosis, whereas relatively high doses of OLE or RUT (>50 μM) inhib...Continue Reading

References

Oct 16, 2003·Molecular and Cellular Biology·Ann-Hwee LeeLaurie H Glimcher
Sep 6, 2006·EMBO Reports·Eva SzegezdiAfshin Samali
Sep 27, 2006·Trends in Neurosciences·Mark P Mattson, Aiwu Cheng
Nov 6, 2007·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Haiwei MengShuwei Liu
Dec 17, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Zohreh GalehdarSean P Cregan
Dec 1, 2012·DNA and Cell Biology·Julia LeitmanGerardo Z Lederkremer
Jan 8, 2013·Neurochemistry International·Juliet M TaylorPeter J Crack
Jan 29, 2013·Trends in Molecular Medicine·Gabriela MercadoClaudio Hetz
Feb 9, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiaotian SunOren A Levy
May 20, 2014·IUBMB Life·Laura HalperinMarek Michalak
Oct 16, 2014·International Journal of Molecular Sciences·Barbara BarbaroClara Balsano
Mar 8, 2016·International Journal of Molecular Sciences·Hafiz Maher Ali ZeeshanHan-Jung Chae
Apr 3, 2016·Brain Research·Navit Ogen-ShternGerardo Z Lederkremer
May 1, 2016·British Journal of Clinical Pharmacology·Daniela PantanoFiorella Casamenti
Jul 28, 2016·Current Pharmaceutical Biotechnology·Miquel MartorellAntoni Sureda
Aug 16, 2016·International Journal of Molecular Sciences·Imène AchourMaria-Grazia Martinoli

❮ Previous
Next ❯

Citations

Oct 24, 2019·International Journal of Molecular Sciences·Bongki ChoYun-Il Lee
Jul 8, 2020·Antioxidants·Han-A Park, Amy C Ellis
Nov 1, 2020·Naunyn-Schmiedeberg's Archives of Pharmacology·Fatemeh ForouzanfarHamid R Sadeghnia

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear translocation

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis