Olf-1-binding site: characterization of an olfactory neuron-specific promoter motif.

Molecular and Cellular Biology
K E KudryckiF L Margolis

Abstract

We report characterization of several domains within the 5' flanking region of the olfactory marker protein (OMP) gene that may participate in regulating transcription of this and other olfactory neuron-specific genes. Analysis by electrophoretic mobility shift assay and DNase I footprinting identifies two regions that contain a novel sequence motif. Interactions between this motif and nuclear proteins were detected only with nuclear protein extracts derived from olfactory neuroepithelium, and this activity is more abundant in olfactory epithelium enriched in immature neurons. We have designated a factor(s) involved in this binding as Olf-1. The Olf-1-binding motif consensus sequence was defined as TCCCC(A/T)NGGAG. Studies with transgenic mice indicate that a 0.3-kb fragment of the OMP gene containing one Olf-1 motif is sufficient for olfactory tissue-specific expression of the reporter gene. Some of the other identified sequence motifs also interact specifically with olfactory nuclear protein extracts. We propose that Olf-1 is a novel, olfactory neuron-specific trans-acting factor involved in the cell-specific expression of OMP.

References

Oct 16, 1992·Brain Research·N S KrishnaM L Getchell
Oct 11, 1991·Brain Research. Molecular Brain Research·D S HowlandL J DeGennaro
Jan 11, 1992·Nucleic Acids Research·S Faisst, S Meyer
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G ThielT C Südhof
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S T SmaleD Baltimore
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S P Jackson, R Tjian
Jan 1, 1989·Annual Review of Biochemistry·P F Johnson, S L McKnight
Nov 1, 1989·Neuron·M KornerY Citri
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E DancigerF Margolis
Jun 5, 1987·Science·T ManiatisJ A Fischer
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K E RogersF L Margolis
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·F L Margolis
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Oct 1, 1984·Archives of Otolaryngology·T NakashimaJ B Snow
Apr 20, 1983·The Journal of Comparative Neurology·R M Costanzo, P P Graziadei

❮ Previous
Next ❯

Citations

Nov 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·E WaltersF L Margolis
Dec 6, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S GarelP Charnay
Feb 4, 1999·Annals of the New York Academy of Sciences·G SicardA Mackay-Sim
Dec 14, 2004·Journal of Biomedical Science·Wen-Kwei ChenAlice Chien Chang
May 13, 2010·Chemical Senses·Timothy S McClintock
Apr 28, 2016·MBio·Helen E FarrellPhilip G Stevenson
Mar 14, 2018·Molecular Neurobiology·Godwin SokporTran Tuoc
Oct 23, 1995·Proceedings. Biological Sciences·G TarozzoA Fasolo
May 1, 1996·Behavior Genetics·H BreerJ Strotmann
Nov 1, 1995·Journal of Neurogenetics·R M Twyman, E A Jones
Mar 22, 2007·The Journal of Biological Chemistry·Anna LagergrenMikael Sigvardsson
Oct 16, 2008·Chemical Senses·Jeremy C McIntyreTimothy S McClintock
Aug 16, 2014·Immunological Reviews·Sören Boller, Rudolf Grosschedl
Nov 5, 2003·The Journal of Biological Chemistry·Sonia MerluzziCarlo Pucillo
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·P Pelosi
Mar 22, 2002·The European Journal of Neuroscience·Arzu CelikS I Korsching
May 16, 2003·Molecular and Cellular Biology·Fang ZhaoTom Kadesch
May 19, 2006·Cellular and Molecular Neurobiology·Danette J NicolayAdil J Nazarali
Oct 24, 2003·Development·Mario Garcia-DominguezPatrick Charnay
Dec 6, 2002·Development·Anna CorradiG Giacomo Consalez
Aug 2, 2001·Journal of Neuroscience Methods·T Wells, D A Carter

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.