Olfactory Performance as an Indicator for Protective Treatment Effects in an Animal Model of Neurodegeneration

Frontiers in Integrative Neuroscience
Anja MeyerMartin Witt

Abstract

Background: Neurodegenerative diseases are often accompanied by olfactory deficits. Here we use a rare neurovisceral lipid storage disorder, Niemann-Pick disease C1 (NPC1), to illustrate disease-specific dynamics of olfactory dysfunction and its reaction upon therapy. Previous findings in a transgenic mouse model (NPC1-/-) showed severe morphological and electrophysiological alterations of the olfactory epithelium (OE) and the olfactory bulb (OB) that ameliorated under therapy with combined 2-hydroxypropyl-ß-cyclodextrin (HPßCD)/allopregnanolone/miglustat or HPßCD alone. Methods: A buried pellet test was conducted to assess olfactory performance. qPCR for olfactory key markers and several olfactory receptors was applied to determine if their expression was changed under treatment conditions. In order to investigate the cell dynamics of the OB, we determined proliferative and apoptotic activities using a bromodeoxyuridine (BrdU) protocol and caspase-3 (cas-3) activity. Further, we performed immunohistochemistry and western blotting for microglia (Iba1), astroglia (GFAP) and tyrosine hydroxylase (TH). Results: The buried pellet test revealed a significant olfactory deterioration in NPC1-/- mice, which reverted to normal levels af...Continue Reading

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Citations

Nov 15, 2018·International Journal of Molecular Sciences·Martin WittAndreas Wree
Sep 11, 2019·International Journal of Molecular Sciences·Anja U BräuerMartin Witt
Feb 22, 2021·Neuroscience and Biobehavioral Reviews·Ariel M Lyons-WarrenBenjamin R Arenkiel
Apr 4, 2021·International Journal of Molecular Sciences·Carsten HolzmannAndreas Wree

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BETA
PCR
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Rad CFX Manager
SPSS
ImageLab
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