PMID: 7520398Jun 1, 1994Paper

Oligoclonal beta-galactoside-binding immunoglobulins antigenically related to 14 kDa lectin in human serum and cerebrospinal fluid: purification and characterization

The International Journal of Biochemistry
R Joubert-CaronD Bladier

Abstract

1. An antiserum raised against a 14 kDa beta-galactoside specific lectin from human brain (HBL14) was used to probe blots from samples of serum and cerebrospinal fluid. The only HBL14-immunoreactive material detected was heavy and light chains of a beta-galactoside-binding IgG fraction (lectin-like IgG). 2. Lectin-like IgG, as well as IgG Fab fragments, compete with HBL14 for binding either to anti-HBL14 antibody or to a lactosyl polyacrylamide-based copolymer. 3. Purification of lectin-like IgG was obtained by affinity chromatography on immobilized rabbit anti-lectin immunoglobulins. The carbohydrate-binding specificity of the purified molecules was restricted to beta-Gal-containing structures and close to the HBL14 one.

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Citations

Feb 1, 1995·Glycoconjugate Journal·A SharmaH J Allen
Mar 1, 1995·Journal of Neuroimmunology·D LutomskiR Joubert-Caron
Jun 27, 1997·Clinica Chimica Acta; International Journal of Clinical Chemistry·D LutomskiM Caron
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·M CaronR Joubert-Caron

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