Oligoclonal IgG and free chains in multiple sclerosis demonstrated by thin-layer polyacrylamide gel isoelectric focusing and immunofixation

Annals of Neurology
M A LaurenziH Link

Abstract

A modified technique of isoelectric focusing on thin-layer polyacrylamide gel followed by immunofixation with monospecific antisera was used to identify individual cerebrospinal fluid (CSF) and serum proteins and to define the oligoclonal reaction observed in multiple sclerosis (MS). "Normal" IgG gave about 20 to 30 bands at pH 3.5 to 9.5, IgA about 10 bands at pH 3.5 to 6.4, beta-trace protein a smear at pH 3.5 to 8.5, and gamma-trace protein 1 or 2 bands at pH 8.0, 9.5 or both. Up to 11 oligoclonal IgG bands migrating between pH 6.5 and 9.5 were found in CSF from 26 of 27 consecutive patients with MS and also in 20 of the corresponding sera, although at lower numbers and concentrations. In 26 patients, 1 or more of the bands corresponding to normal polyclonal IgG were stronger in CSF than in serum. These data support the hypothesis that two colonies of lymphocytes are activated intrathecally, one of them synthesizing oligoclonal and the other polyclonal IgG. Up to 11 mostly faint bands of free light chains, predominantly of lambda type and migrating between pH 3.5 and 9.5, were found in 8 of 9 CSF specimens from patients with MS.

References

Nov 1, 1978·Journal of the Neurological Sciences·A Sidén, K G Kjellin
Aug 1, 1979·Archives of Neurology·A Frydén, H Link
Jan 1, 1979·Clinical Immunology and Immunopathology·C M WeemaesP J van Munster
Apr 1, 1979·Journal of Neurology, Neurosurgery, and Psychiatry·M A Laurenzi, H Link
Oct 1, 1978·Neurology·A C WilliamsD E McFarlin
Jan 1, 1974·Acta Medica Polona·W Cendrowski, K Niedzielska
Oct 1, 1971·Archives of Neurology·H Link, R Müller

❮ Previous
Next ❯

Citations

Jan 1, 1991·Journal of Clinical Laboratory Analysis·G L StanescuR A Rudick
Jun 1, 1987·Journal of Neurology·F BraccoB Tavolato
Sep 1, 1988·Metabolic Brain Disease·T HosokawaE L Hogan
Dec 1, 1982·Italian Journal of Neurological Sciences·P GalloB Tavolato
Oct 1, 1986·Italian Journal of Neurological Sciences·L M GrimaldiR Raffaele
Oct 24, 1998·Acta Neurologica Scandinavica·M KrakauerF Sellebjerg
May 1, 1991·Journal of Neurology, Neurosurgery, and Psychiatry·S FredriksonH Link
Jan 1, 1993·Journal of Neurology, Neurosurgery, and Psychiatry·A ZemanE Thompson
Jun 1, 1981·The Journal of Clinical Investigation·J P PandeyH H Fudenberg
Oct 1, 1994·Journal of Neuroimmunology·C J SindicE C Laterre
Feb 1, 1991·Journal of Neuroimmunology·K FreiH Link
Oct 1, 1986·Journal of Neuroimmunology·P ForsbergH Link
Dec 6, 2011·Immunology Letters·Andrew T HutchinsonRobert L Raison
Feb 1, 1984·Acta Neurologica Scandinavica·J Kinnman, H Link
Jan 1, 1991·Critical Reviews in Clinical Laboratory Sciences·P D Mehta
Jul 24, 2004·Clinical Chemistry·Christian FischerKarl J Lackner
Jul 1, 1986·Annals of Neurology·R A RudickR M Herndon
Jan 1, 1982·Annals of Neurology·R W Cutler, R B Spertell
Jan 1, 1983·Annals of Neurology·J R MillerC T Bever
Apr 1, 1982·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·N S PedersenM Mavra

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.