Oligoclonal T cell expansion in the skin of patients with systemic sclerosis

The Journal of Immunology : Official Journal of the American Association of Immunologists
L I SakkasC D Platsoucas

Abstract

Fibrosis, microvascular fibroproliferative alterations, and autoantibody production are the main features of systemic sclerosis (SSc), and all of them can be explained by cytokine production by activated T cells. However, little is known about the role of T cells in the pathogenesis of SSc, and there is no information on the Ag(s) that elicits such activation. To determine whether T cells infiltrating the skin biopsies of patients with SSc are oligoclonal, beta-chain TCR transcripts from T cells infiltrating the skin of five patients with SSc of recent onset were amplified by either Vbeta-specific PCR or nonpalindromic adaptor PCR. The resulting PCR products were subsequently cloned and sequenced. High proportions of identical beta-chain TCR transcripts ranging from 43 to 90% of those sequenced were found in five patients, strongly suggesting the presence of oligoclonal T cells in these infiltrates. A dominant T cell clone was found to be clonally expanded in skin biopsies obtained from a single patient with SSc at three different times (0, 8, and 13 mo earlier) and from three different skin regions. beta-chain TCR transcripts from PBMC from normal donors (methodological control) were unique when compared with each other, typic...Continue Reading

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Feb 28, 2003·Expert Opinion on Investigational Drugs·Sangeeta D Sule, Fredrick M Wigley
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Jan 13, 2004·Clinical and Diagnostic Laboratory Immunology·Lazaros I SakkasChris D Platsoucas
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