PMID: 7545980Dec 1, 1994Paper

Oligoclonality and skewed T cell receptor V beta gene segment expression in in vivo activated human intestinal intraepithelial T lymphocytes

Immunobiology
G PluschkeK Deusch

Abstract

Intraepithelial intestinal T lymphocytes (IEL) bearing alpha beta or gamma delta T cell receptors (TCR) are positioned to serve as a first line of defense against enteric pathogens. To investigate whether intestinal IEL are subject to antigenic selective forces distinct from that influencing (xp T cells in the peripheral blood (PBL), we performed a comparative analysis of V beta gene segment usage in IEL and PBL of immunologically normal donors by quantitative PCR. Primers for 22 different human TCR V beta gene segments of V beta gene segments families were used to analyze the repertoire of TCR beta chain transcripts in colonic IEL (c-IEL), in corresponding colonic lamina propria lymphocytes (c-LPL), and in peripheral blood lymphocytes. In each of the three individuals examined, a limited number (1-4 out of 22) of TCR V beta families predominated and accounted for more than 50% of the total beta chain transcripts from c-IEL, whereas in PBL and c-LPL a more even distribution of V beta gene families was observed. The dominating V beta gene families were V beta 2, V beta 3, V beta 6, V beta 8 and V beta 14. In one individual, V beta 3 comprised more than 40% of the entire repertoire of c-IEL beta chain transcripts. Sequence analys...Continue Reading

References

Jan 1, 1992·The Journal of Experimental Medicine·C Van KerckhoveM B Brenner
Sep 1, 1990·The Journal of Experimental Medicine·Y ChoiB L Kotzin
Jun 1, 1990·Science·P Marrack, J Kappler
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y W ChoiJ Kappler
Apr 1, 1993·Current Opinion in Immunology·D Guy-Grand, P Vassalli

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Citations

Jul 17, 1996·Journal of Immunological Methods·J A Kearsey, A W Stadnyk
Apr 26, 2006·American Journal of Reproductive Immunology : AJRI·Pouneh DokouhakiMahmood Jeddi-Tehrani

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