Oligodendrocyte degeneration and recovery after focal cerebral ischemia.

Neuroscience
S R McIverM P Goldberg

Abstract

The vulnerability of oligodendrocytes to ischemic injury may contribute to functional loss in diseases of central white matter. Immunocytochemical methods to identify oligodendrocyte injury in experimental models rely on epitope availability, and fail to discriminate structural changes in oligodendrocyte morphology. We previously described the use of a lentiviral vector (LV) carrying enhanced green fluorescent protein (eGFP) under the myelin basic protein (MBP) promoter for selective visualization of oligodendrocyte cell bodies and processes. In this study, we used LV-MBP-eGFP to label oligodendrocytes in rat cerebral white matter prior to transient focal cerebral ischemia, and examined oligodendrocyte injury 24 h, 48 h and 1 week post-reperfusion by quantifying cell survival and assaying the integrity of myelin processes. There was progressive loss of GFP+ oligodendrocytes in ischemic white matter at 24 and 48 h. Surviving GFP+ cells had non-pyknotic nuclear morphology and were terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-negative, but there was marked fragmentation of myelin processes as early as 24 h after stroke. One week after stroke, we observed a restoration of GFP+ oligodendrocytes in ischemic wh...Continue Reading

References

Sep 1, 1996·Stroke; a Journal of Cerebral Circulation·L PantoniJ A Gutierrez
Aug 26, 1998·Journal of Neurobiology·J M García-VerdugoA Alvarez-Buylla
May 29, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·V ValerianiJ McCulloch
Feb 13, 2001·Trends in Neurosciences·J M LevineJ W Fawcett
Jan 5, 2002·Developmental Neuroscience·S BelachewV Gallo
Aug 2, 2002·European Journal of Pharmacology·Carlos MatuteMaría Victoria Sánchez-Gómez
Sep 3, 2002·Journal of Neuroscience Research·Masahiko WatanabeAkiko Nishiyama
Sep 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nathalie Picard-RieraAnne Baron-Van Evercooren
Dec 20, 2002·Developmental Biology·Jeffrey LeeGerard Gradwohl
Jul 5, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·I BadanA Popa-Wagner
Mar 23, 2004·Current Molecular Medicine·Peter K Stys
Apr 23, 2005·Glia·Jennifer K NessMark P Goldberg
Sep 15, 2005·Journal of Neuroscience Research·Sally R McIverMark P Goldberg
Dec 24, 2005·Nature·Ragnhildur KáradóttirDavid Attwell
Apr 25, 2006·Annals of Neurology·S Thomas Carmichael
Jul 28, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bénédicte MennArturo Alvarez-Buylla
Jul 29, 2006·The Journal of Comparative Neurology·Laura L HorkyPhilip J Horner
Dec 15, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John J OhabS Thomas Carmichael
Apr 17, 2007·The European Journal of Neuroscience·Judith M Lytle, Jean R Wrathall
May 12, 2007·Journal of Neuroscience Research·Joshua C MurtieGabriel Corfas
May 17, 2007·Journal of Anatomy·Carlos MatuteFernando Pérez-Cerdá
May 25, 2007·Pharmacology, Biochemistry, and Behavior·Aysan Durukan, Turgut Tatlisumak
Jun 15, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hiroaki MatsumotoJunya Tanaka
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Kunlin Jin, Veronica Galvan
May 3, 2008·Current Opinion in Neurology·Celia Michèle Kassmann, Klaus-Armin Nave
Oct 10, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Leda DimouMagdalena Götz
May 15, 2009·Journal of Neuroscience Methods·Elif G SozmenS Thomas Carmichael

❮ Previous
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Citations

May 23, 2012·Analytical and Bioanalytical Chemistry·Hay-Yan J WangCheng Bin Liu
Mar 1, 2012·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Elif G SozmenS Thomas Carmichael
Jul 8, 2015·Annals of Neurology·Winston D ByblowSuzanne J Ackerley
Apr 30, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Akihiro ShindoKen Arai
Sep 21, 2011·Journal of Neuroscience Research·Devin S GaryJohn W McDonald
Feb 24, 2015·Brain Research·Shira Rosenzweig, S Thomas Carmichael
Mar 25, 2014·Neurochemistry International·Haifa KassisZheng Gang Zhang
Jun 5, 2015·Frontiers in Molecular Neuroscience·Louise C Parr-BrownlieStephanie M Hughes
Jun 28, 2014·Frontiers in Neuroscience·Bilal El WalyPascale Durbec
Dec 17, 2014·Experimental Neurology·Aya D PusicRichard P Kraig
Apr 25, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anna JablonskaPiotr Walczak
May 5, 2017·Annals of Neurology·Qiao-Ling CuiJack P Antel
Jun 9, 2017·Cell Death & Disease·Elisabetta BonfantiMaria P Abbracchio
Jan 27, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Marina Y KhodanovichVasily L Yarnykh
Aug 6, 2015·Stroke; a Journal of Cerebral Circulation·Lijuan HanJun Chen
May 27, 2017·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Bethann M AffeldtAndrea C Pardo
Sep 3, 2020·International Journal of Molecular Sciences·Tatiana Anan'inaMarina Khodanovich
Nov 30, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ian D DuncanLauren A Wierenga
Jan 27, 2019·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Gina M GallucciSuzanne M de la Monte
Dec 14, 2016·Proceedings of the National Academy of Sciences of the United States of America·Elif G SozmenS Thomas Carmichael
Jan 1, 2020·Translational Stroke Research·Shu-Na WangChao-Yu Miao
Jul 23, 2018·The Journal of Steroid Biochemistry and Molecular Biology·Xiaoyan ZhuRachida Guennoun

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