PMID: 8968095Dec 1, 1996Paper

Oligodendrocytes in the adult rat spinal cord undergo radiation-induced apoptosis

Cancer Research
Y Q LiC S Wong

Abstract

Mitotic-linked death is generally regarded as the mode of radiation-induced cell death, particularly in late-responding normal tissues, such as those found in the central nervous system. We have recently reported evidence for radiation-induced apoptosis in the central nervous system using the adult rat spinal cord model. Glial cells, but not neurons or vascular endothelial cells, appeared to undergo apoptosis within 24 h of irradiation. To further characterize the apoptotic process and the type of glial cells involved, a 2-cm segment of the adult rat cervical spinal cord was irradiated with single doses of 1-30 Gy and processed for detailed histological examination at 0, 4, 8, 12, 16, and 24 h after irradiation. Apoptosis was assessed using standard morphological features under the light and electron microscopes and an in situ end labeling assay. A dose response for radiation-induced apoptosis was observed over a dose range of 1-30 Gy, with the peak response at 8 h after irradiation. At 8 h after a 22-Gy irradiation, 96.1% of the apoptotic cells showed positive immunohistochemical staining with Leu-7, a specific marker for oligodendrocytes; only 4.4% of apoptotic cells were positive for Ricinus communis agglutinin-1 (a marker f...Continue Reading

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