Oligodendrocytes produce low molecular weight glycoproteins containing N-acetyl-D-glucosamine in their Golgi apparatus

Glia
M L SuplerW J Streit

Abstract

Lectin histochemistry using the Griffonia simplicifolia II lectin (GSL II) has revealed a novel group of glycoproteins containing terminal N-acetyl-D-glucosamine (GlcNAc) residues in oligodendrocytes. The GlcNAc-containing glycoproteins were not present in other types of glial cells, but were expressed by some neuronal cell populations. Within oligodendrocytes their localization was confined to the Golgi apparatus, as determined ultrastructurally. Biochemical analyses using tricine/SDS-polyacrylamide gel electrophoresis and western blotting with GSL II showed the GlcNAc-containing glycoproteins to be insoluble, with molecular masses ranging from 15 to 30 kDa. Our study provides a first account of insoluble, GlcNAc-rich 15-30 kDa glycoproteins in oligodendroglia. The findings are discussed in the context of the functional significance of other known oligodendrocyte glycoproteins.

References

Jan 1, 1978·Methods in Enzymology·S Ebisu, I J Goldstein
Nov 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W J Streit
Oct 1, 1988·Journal of Neuroscience Research·A Espinosa de los Monteros, J de Vellis
Apr 1, 1989·The Histochemical Journal·T FaraggianaV Marinozzi
Jul 1, 1989·Annals of Neurology·M R Gerber, J R Connor
Apr 1, 1987·Journal of Neurocytology·W J Streit, G W Kreutzberg
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B BlochA Kahn
Oct 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W J StreitS S Spicer
Jan 1, 1983·Developmental Neuroscience·R H Quarles

❮ Previous
Next ❯

Citations

May 1, 1997·Brain Research. Molecular Brain Research·J A Foster, I R Brown

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.