Oligodendroglial apoptosis occurs along degenerating axons and is associated with FAS and p75 expression following spinal cord injury in the rat

Neuroscience
S CashaM G Fehlings

Abstract

Apoptosis or programmed cell death has been reported after CNS trauma. However, the significance of this mechanism in the pathophysiology of spinal cord injury, in particular at the cervical level, requires further investigation. In the present study, we used the extradural clip compression model in the rat to examine the cellular distribution of apoptosis following cervical spinal cord injury, the relationship between glial apoptosis and post-traumatic axonal degeneration and the possible role of apo[apoptosis]-1, CD95 (FAS) and p75 in initiating post-traumatic glial apoptosis. In situ terminal-deoxy-transferase mediated dUTP nick end labeling revealed apoptotic cells, largely oligodendrocytes as identified by cell specific markers, in grey and white matter following spinal cord injury. Apoptotic cell death was confirmed using electron microscopy and by the demonstration of DNA laddering on agarose gel electrophoresis. Beta-amyloid precursor protein was used as a molecular marker of axonal degeneration on western blots and immunohistochemistry. Degeneration of axons was temporally and spatially co-localized with glial apoptosis. FAS and p75 protein expression was seen in astrocytes, oligodendrocytes and microglia, and was also...Continue Reading

References

Feb 1, 1976·Journal of the Neurological Sciences·I R Griffiths
Dec 31, 1991·Biochemical and Biophysical Research Communications·B DipasqualeR J Youle
Jul 1, 1988·The Journal of the American Paraplegia Society·A Blight
Jan 1, 1988·Neurosurgery·P BlackM Feldman
Jun 1, 1973·Teratology·J U Schweichel, H J Merker
May 16, 1984·Biochemical and Biophysical Research Communications·G G Glenner, C W Wong
Mar 10, 1995·Science·S Nagata, P Golstein
May 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B Grasl-KrauppR Schulte-Hermann
Aug 30, 1994·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D P LaneP A Hall
Jan 1, 1994·Acta Neuropathologica·F E SherriffS Wilson
Jul 16, 1993·Science·S RabizadehD E Bredesen
Mar 1, 1996·Journal of Neuropathology and Experimental Neurology·G L LiY Olsson
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·S O YoonM V Chao
Dec 1, 1996·The Journal of Experimental Medicine·S D D'SouzaJ P Antel
Jan 20, 1997·The Journal of Comparative Neurology·P G PopovichB T Stokes
Jan 31, 1997·The Journal of Biological Chemistry·M MuzioV M Dixit
Feb 1, 1997·Journal of Neurotrauma·S K Agrawal, M G Fehlings
Jan 7, 1998·Journal of Neuroscience Research·S L ShumanM S Beattie
Apr 8, 1998·Trends in Neurosciences·B BecherJ P Antel
May 15, 1998·Neuron·B Pettmann, C E Henderson

❮ Previous
Next ❯

Citations

Aug 3, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Stefan Klussmann, Ana Martin-Villalba
Nov 30, 2005·Anatomy and Embryology·Katarina VilovićMirna Saraga-Babić
Sep 22, 2011·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Kenzo UchidaHisatoshi Baba
Jan 26, 2008·Cellular and Molecular Neurobiology·Xin LiAiguo Shen
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Suman JayadevGwenn A Garden
Mar 2, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Janelle M FassbenderTheo Hagg
Mar 16, 2011·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Akshata AlmadDana M McTigue
Jan 24, 2004·Brain Research. Brain Research Reviews·Chulhee Choi, Etty N Benveniste
May 18, 2005·Molecular and Cellular Neurosciences·Ana MingoranceJosé A Del Río
Apr 2, 2004·Neurobiology of Disease·Christos ProfyrisSteven Petratos
Apr 2, 2004·Neurobiology of Disease·Cindy ZehrMike Hutton
Jan 30, 2004·The Spine Journal : Official Journal of the North American Spine Society·Nicholas C Bambakidis, Robert H Miller
May 10, 2003·Brain Research. Brain Research Reviews·Swapan K RayNaren L Banik
Jul 9, 2002·Journal of Neuroscience Methods·Raad NashmiMichael G Fehlings
Aug 10, 2002·Progress in Neurobiology·Philippe P Roux, Philip A Barker
Sep 30, 2008·Chinese Journal of Traumatology = Zhonghua Chuang Shang Za Zhi·Bo Wu, Xian-jun Ren
Jul 3, 2003·Molecular and Cellular Neurosciences·Christopher B McBrideJohn D Steeves
Nov 15, 2002·Brain Research. Brain Research Protocols·Andrei V KrassioukovMichael G Fehlings
Nov 12, 2003·The Spine Journal : Official Journal of the North American Spine Society·David H KimEdward C Benzel
Apr 2, 2004·Nature Medicine·Catherine Barthélémy, Christopher E Henderson
Aug 24, 2004·Nature Reviews. Neuroscience·Susanna C Benn, Clifford J Woolf
Apr 25, 2006·Journal of Neurotrauma·Theo Hagg, Martin Oudega
Apr 25, 2006·Journal of Neurotrauma·Lisa McKerracher, Haruhisa Higuchi
Apr 25, 2006·Journal of Neurotrauma·Darryl C Baptiste, Michael G Fehlings
Apr 25, 2006·Journal of Neurotrauma·Robert W KeaneW Dalton Dietrich
Apr 26, 2008·Journal of Neurotrauma·James W Austin, Michael G Fehlings
May 8, 2013·Journal of Neurotrauma·Jian-Zhong HuHong-Bin Lu
Nov 27, 2009·Stem Cells and Development·Francisco J RiveraLudwig Aigner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis