Oligo(ethylene glycol)-Functionalized Squaraine Fluorophore as a Near-Infrared-Fluorescent Probe for the In Vivo Detection of Diagnostic Enzymes

Analytical Chemistry
Bo WuShuizhu Wu

Abstract

Squaraine dyes have excellent photostability with intensive absorption and strong fluorescence in the near-infrared (NIR) region. However, they display a strong tendency to aggregate in aqueous media because of their poor water solubility, often causing fluorescence quenching that severely limits their in vivo applications, especially for detecting or imaging diagnostic enzymes. In this work, an oligo(ethylene glycol)-functionalized squaraine fluorophore has been developed as an NIR-fluorescent probe that can detect and image the activities of a diagnostic enzyme (leucine aminopeptidase) both in vitro and in vivo. The probe shows near-infrared absorption and emission, a low detection limit (0.61 ng/mL), relatively good aqueous solubility, high selectivity, and little toxicity. In addition, the probe herein was successfully used to track endogenous leucine aminopeptidase both in vitro and in vivo with a nude-mouse model.

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Citations

Jul 19, 2019·Analytical Chemistry·Jianguo WangBen Zhong Tang
Dec 19, 2019·The Journal of Organic Chemistry·Futa OgawaMasaaki Omote

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